Centrin is essential for the activity of the ciliary reversal-coupled voltage-gated Ca2+ channels

Biochemical and Biophysical Research Communications
Kohsuke GondaMihoko Takahashi

Abstract

Voltage-gated Ca(2+) channels play a critical role in controlling Ca(2+) entry in various cells. Ciliary reversal in Paramecium depends on the Ca(2+) influx through voltage-gated Ca(2+) channels on the ciliary membrane. One of the voltage-gated Ca(2+) channel mutants in Paramecium caudatum, cnrC, neither produces Ca(2+) action potentials nor responds to any depolarizing stimuli. Here, we report that the cnrC(+) gene product is P. caudatum centrin (Pccentrin1p), a member of the Ca(2+)-binding EF-hand protein superfamily. The Pccentrin1p gene of cnrC was found to contain a single-base deletion, a mutation that caused the loss of the fourth EF-hand of Pccentrin1p. Moreover, the wild-type Ca(2+) channel function was impaired by Pccentrin1p gene silencing, leading to the loss of current-evoked Ca(2+) action potentials and stimulated ciliary reversal. These results demonstrate that Pccentrin1p is indispensable for the activity of the voltage-gated Ca(2+) channels that control ciliary reversal in Paramecium.

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Citations

Sep 29, 2011·Journal of Fluorescence·Ya-Qin ZhaoBin-Sheng Yang
Jun 6, 2006·Journal of the American Society for Mass Spectrometry·Theodore A CraigRajiv Kumar
Jul 9, 2005·Eukaryotic Cell·Stefan Geimer, Michael Melkonian
Jul 27, 2011·Journal of Photochemistry and Photobiology. B, Biology·Yaqin ZhaoBinsheng Yang
Jan 9, 2008·Cell Calcium·Yoshiaki Iwadate, Yasuo Nakaoka
Sep 30, 2015·Plant Signaling & Behavior·Hiroshi TakaichiTomonori Kawano
Mar 6, 2008·The Journal of Eukaryotic Microbiology·Eric D RobinetteHeather G Kuruvilla
Apr 20, 2006·Journal of Theoretical Biology·Naoko OgawaMasatoshi Ishikawa
Aug 19, 2007·Biochemical and Biophysical Research Communications·Kohsuke GondaMihoko Takahashi
Dec 8, 2005·Current Biology : CB·Françoise RuizFrance Koll
Oct 28, 2009·Journal of Molecular Biology·Juan Martinez-SanzConstantin T Craescu
Sep 2, 2017·Physical Biology·Eun Gul Chung, Sangjin Ryu
Nov 9, 2017·Metallomics : Integrated Biometal Science·Enxian ShiBinsheng Yang
Jun 6, 2006·Molecular Biology and Evolution·Kisaburo Nagamune, L David Sibley
Dec 7, 2007·Journal of Cell Science·Delphine GogendeauJanine Beisson

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