Centriolar SAS-5 is required for centrosome duplication in C. elegans

Nature Cell Biology
Marie DelattrePierre Gönczy

Abstract

Centrosomes, the major microtubule-organizing centres (MTOCs) of animal cells, are comprised of a pair of centrioles surrounded by pericentriolar material (PCM). Early in the cell cycle, there is a single centrosome, which duplicates during S-phase to direct bipolar spindle assembly during mitosis. Although crucial for proper cell division, the mechanisms that govern centrosome duplication are not fully understood. Here, we identify the Caenorhabditis elegans gene sas-5 as essential for daughter-centriole formation. SAS-5 is a coiled-coil protein that localizes primarily to centrioles. Fluorescence recovery after photobleaching (FRAP) experiments with green fluorescent protein (GFP) fused to SAS-5 (GFP-SAS-5) demonstrated that the protein shuttles between centrioles and the cytoplasm throughout the cell cycle. Analysis of mutant alleles revealed that the presence of SAS-5 at centrioles is crucial for daughter-centriole formation and that ZYG-1, a kinase that is also essential for this process, controls the distribution of SAS-5 to centrioles. Furthermore, partial RNA-interference (RNAi)-mediated inactivation experiments suggest that both sas-5 and zyg-1 are dose-dependent regulators of centrosome duplication.

References

May 1, 1978·Journal of Ultrastructure Research·N WolfJ R McIntosh
Mar 1, 1992·Journal of Structural Biology·M PaintrandM Bornens
Dec 1, 1981·The Journal of Cell Biology·R Kuriyama, G G Borisy
May 1, 1993·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·D G Albertson, J N Thomson
Feb 7, 1998·Journal of Structural Biology·D ChrétienE Karsenti
Jan 4, 2001·Current Biology : CB·F PianoK J Kemphues
Aug 15, 2002·Current Biology : CB·Jeffrey L SalisburyMargaret Springett
Nov 14, 2002·Molecular Biology of the Cell·Susan K DutcherJohn Stanga
Jan 3, 2003·Nature Cell Biology·Paul ChangTim Stearns
Feb 26, 2003·Cell·Matthew KirkhamAnthony A Hyman
May 21, 2003·Development·Laura D MathiesJudith Kimble
Jul 15, 2003·Molecular Biology of the Cell·Anne PaolettiMichel Bornens
Sep 6, 2003·Current Biology : CB·Jean Michel Bellanger, Pierre Gönczy
Apr 8, 2004·Developmental Cell·Catherine A KempKevin F O'Connell

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Citations

Mar 27, 2010·Cellular and Molecular Life Sciences : CMLS·Thomas Müller-ReichertMartin Srayko
Jun 14, 2012·Nature Reviews. Molecular Cell Biology·Pierre Gönczy
Feb 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Mara SchvarzsteinAnne M Villeneuve
Jun 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Manuel HilbertIoannis Vakonakis
Sep 24, 2010·Molecular Biology of the Cell·Christopher KasbekHarold A Fisk
Oct 29, 2010·Molecular Biology of the Cell·Ching-Hui YangHarold A Fisk
Aug 7, 2009·Genes & Development·Alexander DammermannKaren Oegema
Feb 11, 2010·Journal of Cell Science·Nathaniel PetersKevin F O'Connell
Jul 23, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Michel Bornens, Pierre Gönczy
Jul 23, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Masafumi Hirono
Jul 23, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Elif Nur Fırat-Karalar, Tim Stearns
Apr 20, 2011·Cold Spring Harbor Symposia on Quantitative Biology·E Hatch, T Stearns
Aug 16, 2012·Cellular and Molecular Life Sciences : CMLS·Christopher W Brownlee, Gregory C Rogers
Dec 2, 2009·The Journal of Cell Biology·Naomi R StevensJordan W Raff
Feb 4, 2010·The Journal of Cell Biology·Naomi R StevensJordan W Raff
Jul 16, 2008·Trends in Cell Biology·Petr Strnad, Pierre Gönczy
Jun 9, 2009·The Plant Journal : for Cell and Molecular Biology·Hua JiangZhong-Nan Yang
May 7, 2009·Traffic·Inês Cunha-FerreiraMónica Bettencourt-Dias
Jun 15, 2007·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Susan K Dutcher
Mar 24, 2016·The Journal of Cell Biology·Marie-Hélène Verlhac
Feb 24, 2005·European Journal of Cell Biology·Cornelia Schmutz, Anne Spang
Dec 9, 2014·Cell Cycle·Ahuvit DavidAlwin Krämer
Dec 4, 2012·Current Opinion in Cell Biology·Tomer Avidor-Reiss, Jayachandran Gopalakrishnan
Jun 24, 2015·The Journal of Cell Biology·Tyler C MoyerAndrew J Holland
May 12, 2009·Current Biology : CB·Steffen LawoLaurence Pelletier
Jun 2, 2009·Current Biology : CB·Thorsten I SchmidtErich A Nigg
Sep 19, 2006·Current Biology : CB·Marie DelattrePierre Gönczy
Sep 28, 2010·Current Biology : CB·Juliette Azimzadeh, Wallace F Marshall
Oct 9, 2008·Current Opinion in Cell Biology·Mi Hye SongKevin F O'Connell
Jan 12, 2010·Developmental Cell·Daiju KitagawaPierre Gönczy
Dec 15, 2010·Developmental Cell·Naomi R StevensJordan W Raff
May 20, 2014·Current Biology : CB·Zsofia A NovakJordan W Raff

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