Centrosome/spindle pole-associated protein regulates cytokinesis via promoting the recruitment of MyoGEF to the central spindle.

Molecular Biology of the Cell
M AsieduQ Wei

Abstract

Cooperative communications between the central spindle and the contractile ring are critical for the spatial and temporal regulation of cytokinesis. Here we report that MyoGEF, a guanine nucleotide exchange factor that localizes to the central spindle and cleavage furrow, interacts with centrosome/spindle pole-associated protein (CSPP), which is concentrated at the spindle pole and central spindle during mitosis and cytokinesis. Both in vitro and in vivo pulldown assays show that MyoGEF interacts with CSPP. The C-terminus of MyoGEF and N-terminus of CSPP are required for their interaction. Immunofluorescence analysis indicates that MyoGEF and CSPP colocalize at the central spindle. Depletion of CSPP or MyoGEF by RNA-interference (RNAi) not only causes defects in mitosis and cytokinesis, such as metaphase arrest and furrow regression, but also mislocalization of nonmuscle myosin II with a phosphorylated myosin regulatory light chain (p-MRLC). Importantly, CSPP depletion by RNAi interferes with MyoGEF localization at the central spindle. Finally, MyoGEF interacts with ECT2, and RNAi-mediated depletion of MyoGEF leads to mislocalization of ECT2 and RhoA during cytokinesis. Therefore, we propose that CSPP interacts with and recruit...Continue Reading

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Citations

Jul 17, 2010·The Journal of Biological Chemistry·Di WuQize Wei
Jun 4, 2010·Molecular Biology of the Cell·Sebastian PatzkeTrond Stokke
Jul 26, 2013·Molecular Biology of the Cell·Matthew K MartzPhilip B Wedegaertner
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Apr 24, 2010·The International Journal of Biochemistry & Cell Biology·Yi-Wen Heng, Cheng-Gee Koh
Jan 7, 2018·Cellular and Molecular Life Sciences : CMLS·Kiet Hua, Russell J Ferland
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Jan 29, 2020·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Juntao KeXiaoping Miao
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