Cepharanthine exerts antitumor activity on choroidal melanoma by reactive oxygen species production and c-Jun N-terminal kinase activation

Oncology Letters
Qi ZhuLing Zhang

Abstract

Choroidal melanoma is the most common primary intraocular tumor in adults. Cepharanthine (CEP), a natural alkaloid extracted from the roots of Stephania cepharantha Hayata, has been demonstrated to inhibit the proliferation of various cancer cells. However, its potential anticancer effect in choroidal melanoma has not been clarified yet. In the present study, it was identified that CEP may potently inhibit the proliferation of human choroidal melanoma cells, induce cell death and cell cycle arrest, and activate cellular apoptotic proteins, including Bcl-2-associated X protein, caspase and poly(ADP-ribose) polymerase. Results also revealed that CEP induced the cellular production of reactive oxygen species (ROS) and led to cytochrome c release, whereas concurrent treatment with N-acetyl-L-cysteine (a ROS scavenger) attenuated the situation. In addition, CEP was also revealed to activate c-Jun N-terminal kinase (JNK) 1 and 2, whereas inhibition of JNK1/2 partially abrogated the proliferation inhibitory effect of CEP, indicating that JNK1 and JNK2 were involved in CEP-triggered cellular apoptosis. In addition, the anticancer effects of CEP were also observed in a choroidal melanoma xenograft model. In summary, the results of the p...Continue Reading

References

Mar 1, 1991·Ophthalmology·E S GragoudasM D Spar
May 1, 1997·Ophthalmology·P T Finger
Dec 24, 1997·Survey of Ophthalmology·P T Finger
Mar 6, 1998·Archives of Ophthalmology·J A OosterhuisJ E Keunen
May 30, 1998·Methods and Findings in Experimental and Clinical Pharmacology·S FurusawaY Takayanagi
Jan 23, 1999·Cell Death and Differentiation·B SingleP Nicotera
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·B L BennettD W Anderson
Nov 1, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Petra RietschelPaul B Chapman
Dec 13, 2005·Cell Death and Differentiation·H NakanoK Okumura
Mar 17, 2006·Free Radical Biology & Medicine·Han-Ming Shen, Zheng-gang Liu
Oct 22, 2008·Oncogene·D N Dhanasekaran, E P Reddy
Jan 20, 2009·Biochemical Pharmacology·Ying ZhouZhe-Sheng Chen
Dec 25, 2009·Journal of Enzyme Inhibition and Medicinal Chemistry·Ilhami GülçinFevzi Topal
Apr 24, 2010·Cancer Science·Wunchana SeubwaiSopit Wongkham
May 24, 2011·Pharmacological Reports : PR·Moshe Rogosnitzky, Rachel Danks
Jun 28, 2011·Ophthalmology·Arun D SinghAllan K Topham
May 5, 2012·Oman Journal of Ophthalmology·Parul Singh, Abhishek Singh
Dec 21, 2013·Nature Reviews. Molecular Cell Biology·Peter E CzabotarJerry M Adams

❮ Previous
Next ❯

Citations

Feb 29, 2020·Expert Opinion on Therapeutic Targets·Yao WangYi-Fei Wang
May 23, 2018·Oxidative Medicine and Cellular Longevity·Teruaki TakasakiReiko Sugiura
Apr 28, 2020·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Yueping JiangShao Liu
May 28, 2019·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Christian Bailly

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.