Abstract
Choroidal melanoma is the most common primary intraocular tumor in adults. Cepharanthine (CEP), a natural alkaloid extracted from the roots of Stephania cepharantha Hayata, has been demonstrated to inhibit the proliferation of various cancer cells. However, its potential anticancer effect in choroidal melanoma has not been clarified yet. In the present study, it was identified that CEP may potently inhibit the proliferation of human choroidal melanoma cells, induce cell death and cell cycle arrest, and activate cellular apoptotic proteins, including Bcl-2-associated X protein, caspase and poly(ADP-ribose) polymerase. Results also revealed that CEP induced the cellular production of reactive oxygen species (ROS) and led to cytochrome c release, whereas concurrent treatment with N-acetyl-L-cysteine (a ROS scavenger) attenuated the situation. In addition, CEP was also revealed to activate c-Jun N-terminal kinase (JNK) 1 and 2, whereas inhibition of JNK1/2 partially abrogated the proliferation inhibitory effect of CEP, indicating that JNK1 and JNK2 were involved in CEP-triggered cellular apoptosis. In addition, the anticancer effects of CEP were also observed in a choroidal melanoma xenograft model. In summary, the results of the p...Continue Reading
References
Jun 1, 1991·Japanese Journal of Pharmacology·H ItoH Noda
Mar 1, 1991·Ophthalmology·E S GragoudasM D Spar
Jan 1, 1994·Cancer Chemotherapy and Pharmacology·M OnoK Orita
Oct 1, 1996·Cancer Letters·T HottaH Terasawa
May 1, 1997·Ophthalmology·P T Finger
Dec 24, 1997·Survey of Ophthalmology·P T Finger
Mar 6, 1998·Archives of Ophthalmology·J A OosterhuisJ E Keunen
May 30, 1998·Methods and Findings in Experimental and Clinical Pharmacology·S FurusawaY Takayanagi
Jan 23, 1999·Cell Death and Differentiation·B SingleP Nicotera
Aug 12, 1999·Ophthalmology·M W Wilson, J L Hungerford
Aug 31, 2001·Journal of Cellular Biochemistry·J WuI Nakashima
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·B L BennettD W Anderson
Sep 18, 2002·Kidney International·Bin YangA Meguid El Nahas
Nov 1, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Petra RietschelPaul B Chapman
Dec 13, 2005·Cell Death and Differentiation·H NakanoK Okumura
Jan 18, 2006·FEBS Letters·Kamal Krishna BiswasIkuro Maruyama
Mar 17, 2006·Free Radical Biology & Medicine·Han-Ming Shen, Zheng-gang Liu
Jan 6, 2007·Life Sciences·Shinobu Furusawa, Jianghong Wu
Apr 30, 2008·Cancer Biology & Therapy·Masamichi ItaZbigniew Darzynkiewicz
Oct 22, 2008·Oncogene·D N Dhanasekaran, E P Reddy
Jan 20, 2009·Biochemical Pharmacology·Ying ZhouZhe-Sheng Chen
Dec 25, 2009·Journal of Enzyme Inhibition and Medicinal Chemistry·Ilhami GülçinFevzi Topal
Apr 24, 2010·Cancer Science·Wunchana SeubwaiSopit Wongkham
May 24, 2011·Pharmacological Reports : PR·Moshe Rogosnitzky, Rachel Danks
Jun 4, 2011·Journal of Signal Transduction·Yong SonHyun-Ock Pae
Jun 28, 2011·Ophthalmology·Arun D SinghAllan K Topham
Jan 4, 2012·Acta Pharmacologica Sinica·Zan ChenMing Xu
May 5, 2012·Oman Journal of Ophthalmology·Parul Singh, Abhishek Singh
Dec 21, 2013·Nature Reviews. Molecular Cell Biology·Peter E CzabotarJerry M Adams
Citations
Feb 29, 2020·Expert Opinion on Therapeutic Targets·Yao WangYi-Fei Wang
May 23, 2018·Oxidative Medicine and Cellular Longevity·Teruaki TakasakiReiko Sugiura
Nov 14, 2019·Chinese Medicine·Hua LuoYitao Wang
Apr 28, 2020·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Yueping JiangShao Liu
May 28, 2019·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Christian Bailly
Aug 5, 2020·Anti-cancer Agents in Medicinal Chemistry·Yue ZhaoJianhong Yang