Cepharanthine induces apoptosis through reactive oxygen species and mitochondrial dysfunction in human non-small-cell lung cancer cells

Biochemical and Biophysical Research Communications
Peiyan HuaXingyi Zhang

Abstract

Cepharanthine is a medicinal plant-derived natural compound which possesses potent anti-cancer properties. However, there is little report about its effects on lung cancer cells. In this study, we investigated the effects of cepharanthine on the cell viability and apoptosis in human non-small-cell lung cancer H1299 and A549 cells. It was found that cepharanthine inhibited the growth of H1299 and A549 cells in a dose-dependent manner which was associated with the generation of reactive oxygen species(ROS) and the dissipation of mitochondrial membrane potential (Δψm). These effects were markedly abrogated when cells were pretreated with N-acetylcysteine (NAC), a specific ROS inhibitor, indicating that the apoptosis-inducing effect of cepharanthine in lung cancer cells was mediated by ROS. In addition, cepharanthine triggered apoptosis in non-small lung cancer cells via the upregulation of Bax, downregulation of Bcl-2 and significant activation of caspase-3 and PARP. These results provide the rationale for further research and preclinical investigation of cepharanthine's anti-tumor effect against human non-small-cell lung cancer.

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Citations

Nov 8, 2015·Biochemical and Biophysical Research Communications·Ming ZhanJian Wang
Apr 15, 2016·Journal of Biochemical and Molecular Toxicology·Peiyan HuaBingjin Li
Apr 29, 2016·Immunopharmacology and Immunotoxicology·Tomofumi UtoMasanori Baba
Mar 30, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Poorna Chandra RaoD Saketh Sriram
Jun 8, 2018·Current Medicinal Chemistry·Tijana StankovićMilica Pešić
Feb 29, 2020·Expert Opinion on Therapeutic Targets·Yao WangYi-Fei Wang
Jul 12, 2019·Phytotherapy Research : PTR·Onsurang WattanathamsanVarisa Pongrakhananon
May 10, 2019·Journal of Experimental & Clinical Cancer Research : CR·Hsin-Ling YangYou-Cheng Hseu
Jan 30, 2019·Journal of Asian Natural Products Research·Sukanya UnsonPiyanuch Wonganan
Apr 28, 2020·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Yueping JiangShao Liu
Feb 11, 2016·Molecular Cancer Therapeutics·Hee Dong HanJeong-Won Lee
May 28, 2019·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Christian Bailly
Jul 24, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jyoti SinghAbha Meena
Jan 24, 2020·Journal of Pharmacopuncture·Hong Jae KimSu Hyun Hong

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