Ceramide and Toll-like receptor 4 are mobilized into membrane rafts in response to Helicobacter pylori infection in gastric epithelial cells.

Infection and Immunity
Dah-Yuu LuChih-Ho Lai

Abstract

Helicobacter pylori infection is thought to be involved in the development of several gastric diseases. Two H. pylori virulence factors (vacuolating cytotoxin A and cytotoxin-associated gene A) reportedly interact with lipid rafts in gastric epithelial cells. The role of Toll-like receptor (TLR)-mediated signaling in response to H. pylori infection has been investigated extensively in host cells. However, the receptor molecules in lipid rafts that are involved in H. pylori-induced innate sensing have not been well characterized. This study investigated whether lipid rafts play a role in H. pylori-induced ceramide secretion and TLR4 expression and thereby contribute to inflammation in gastric epithelial cells. We observed that both TLR4 and MD-2 mRNA and protein levels were significantly higher in H. pylori-infected AGS cells than in mock-infected cells. Moreover, significantly more TLR4 protein was detected in detergent-resistant membranes extracted from H. pylori-infected AGS cells than in those extracted from mock-infected cells. However, this effect was attenuated by the treatment of cells with cholesterol-usurping agents, suggesting that H. pylori-induced TLR4 signaling is dependent on cholesterol-rich microdomains. Similar...Continue Reading

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Citations

May 7, 2013·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Yung-An TsouHui-Chen Chen
Jun 21, 2015·Journal of Bioscience and Bioengineering·Chun-Jung LinChih-Ho Lai
Mar 19, 2014·Nanomedicine·Chia-Der LinChih-Ho Lai
Feb 23, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Chun-Jung LinYew-Min Tzeng
Apr 16, 2015·Mediators of Inflammation·Aline Cristina Targa CadamuroAna Elizabete Silva
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Aug 8, 2017·International Journal of Molecular Sciences·Fu-Chen Huang
Feb 2, 2017·Frontiers in Cellular and Infection Microbiology·Wei-Chih LiaoChih-Ho Lai
Jan 17, 2019·Journal of Clinical Medicine·Yi-Hsing ChenChih-Ho Lai
Aug 2, 2018·Journal of Mammary Gland Biology and Neoplasia·Xiaoyu HuYunhe Fu
Mar 16, 2018·Mediators of Inflammation·Sabine GröschElisabetta Albi

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