Ceramide galactosyltransferase (UGT8) is a molecular marker of breast cancer malignancy and lung metastases.

British Journal of Cancer
Piotr DziegielMaciej Ugorski

Abstract

It was shown recently on the level of gene expression that UGT8, coding UDP-galactose:ceramide galactosyltransferase, is one of six genes whose elevated expression correlated with a significantly increased the risk of lung metastases in breast cancer patients. In this study primary tumours and their lung metastases as well as breast cancer cell lines were analysed for UGT8 expression at the protein level. Expression of UGT8 in breast cancer tissue specimens and breast cancer cell lines was analysed using IHC, real-time PCR and Western blotting. Comparison of the average values of the reaction intensities (IRS scale) showed a significant difference in UGT8 expression between (1) primary and metastatic tumours (Mann-Whitney U, P<0.05), (2) tumours of malignancy grades G3 and G2 (Mann-Whitney U, P<0.01) as well as G3 and G1 (Mann-Whitney U, P<0.001) and (3) node-positive and node-negative tumours (Mann-Whitney U, P<0.001). The predictive ability of increased expression of UGT8 was validated at the mRNA level in three independent cohorts of breast cancer patients (721). Similarly, breast cancer cell lines with the 'luminal epithelial-like' phenotype did not express or weakly expressed UGT8, in contrast to malignant, 'mesenchymal-li...Continue Reading

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Citations

Sep 7, 2017·Molecular & Cellular Proteomics : MCP·Kangling ZhangLawrence C Sowers
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Apr 23, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zibo LiCaiyan Wang

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Methods Mentioned

BETA
PCR
protein
electrophoresis
acetylation

Software Mentioned

AnalySis
Statisitica

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