Ceramide generation occurring during 7beta-hydroxycholesterol- and 7-ketocholesterol-induced apoptosis is caspase independent and is not required to trigger cell death

Cell Death and Differentiation
C MiguetG Lizard

Abstract

Biological activities of oxysterols seem tightly regulated. Therefore, the ability to induce cell death of structurally related oxysterols, such as those oxidized at C7(7alpha-, 7beta-hydroxycholesterol, and 7-ketocholesterol), was investigated on U937 cells at different times of treatment in a concentration range of 5-80 microg/ml. Whereas all oxysterols accumulate inside the cells, strong inhibition of cell growth and increased permeability to propidium iodide were observed only with 7beta-hydroxycholesterol and 7-ketocholesterol, which trigger an apoptotic process characterized by the occurrence of cells with fragmented and/or condensed nuclei, and by various cellular dysfunctions: loss of mitochondrial transmembrane potential, cytosolic release of cytochrome c, activation of caspase-9 and -3 with subsequent enhanced activity of caspase-3, degradation of poly(ADP-ribose) polymerase, and increased accumulation of cellular C16 : 0 and C24 : 1 ceramide species. This ceramide generation is not attributed to caspase activation since inhibition of 7beta-hydroxycholesterol- and 7-ketocholesterol-induced apoptosis by Z-VAD-fmk (100 microM), a broad spectrum caspase inhibitor, did not reduce C16 : 0 and C24 : 1 ceramide species accum...Continue Reading

References

Oct 15, 1977·Experimental Cell Research·R CornellA F Horwitz
Jan 1, 1988·Annual Review of Cell Biology·L B Chen
Oct 1, 1988·Journal of Steroid Biochemistry·C B Lazier, B V Bapat
Jul 1, 1987·Chemistry and Physics of Lipids·L L Smith
Oct 1, 1986·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·P B Addis
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Feb 1, 1995·Trends in Biochemical Sciences·Y A Hannun, L M Obeid
Feb 3, 1995·The Journal of Biological Chemistry·S JayadevY A Hannun
Apr 1, 1994·Trends in Biochemical Sciences·G de Murcia, J Ménissier de Murcia
Feb 1, 1996·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·F GuardiolaJ Boatella
May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·J T PaiJ L Goldstein
Jan 1, 1996·Free Radical Biology & Medicine·J A Berliner, J W Heinecke
Nov 1, 1995·Immunobiology·K AupeixP Bischoff
Sep 24, 1996·Biochemical and Biophysical Research Communications·E Nishio, Y Watanabe
Dec 1, 1996·Endocrinology·J P WittyA L Johnson
Feb 7, 1997·The Journal of Biological Chemistry·J M LehmannT M Willson
Mar 15, 1997·Genes & Development·S G KennedyN Hay
Apr 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A S AroraG J Gores
May 22, 1998·Neurochemistry International·J Y Chang, L Z Liu
Aug 26, 1998·Trends in Cell Biology·D Green, G Kroemer
Aug 26, 1998·Biochimica Et Biophysica Acta·J CaiD P Jones
Aug 26, 1998·Biochimica Et Biophysica Acta·S A SusinG Kroemer

❮ Previous
Next ❯

Citations

Mar 31, 2004·Molecular Aspects of Medicine·Gabriella LeonarduzziGiuseppe Poli
May 22, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Y C O'CallaghanN M O'Brien
Jan 29, 2003·The Journal of Nutritional Biochemistry·Gabriella LeonarduzziGiuseppe Poli
Jun 9, 2009·Photochemistry and Photobiology·Ignacio R Rodriguez, Steven J Fliesler
Apr 24, 2007·Free Radical Biology & Medicine·Paola PalozzaFranco O Ranelletti
Sep 5, 2013·Free Radical Research·G ServiddioG Vendemiale
Apr 25, 2007·Current Eye Research·Corinne JoffreLionel Bretillon
Mar 31, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·K KoschutnigK-H Wagner
Mar 10, 2016·Chemistry and Physics of Lipids·Waldemar KuligIlpo Vattulainen
Mar 3, 2004·Journal of Biochemical and Molecular Toxicology·Lisa RyanNora M O'Brien
Apr 11, 2006·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Céline PrunetGérard Lizard
Oct 12, 2014·Ageing Research Reviews·Amira ZarroukGérard Lizard
Aug 23, 2008·Free Radical Biology & Medicine·Jing ChenClayton E Mathews
Feb 22, 2005·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Y W ChengC F Wang
May 23, 2009·Toxicology·Richard R Chapleau, Martin Sagermann
Jul 5, 2005·Biochemical and Biophysical Research Communications·Hyun Sun LeeKoanhoi Kim
Aug 29, 2006·Free Radical Biology & Medicine·David A LarssonWei Li
Apr 26, 2011·Chemistry and Physics of Lipids·John J Mackrill
Nov 22, 2016·PloS One·Stefan WallnerGerd Schmitz
Aug 23, 2008·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·A VejuxG Lizard
Jul 12, 2018·Critical Reviews in Food Science and Nutrition·Fatiha BrahmiGérard Lizard
Dec 14, 2004·The Journal of Biological Chemistry·Thomas J Nelson, Daniel L Alkon
Feb 7, 2019·Physiological Reviews·Robyn MeechPeter I Mackenzie

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis