Ceramide-induced BOK promotes mitochondrial fission in preeclampsia

Cell Death & Disease
Jonathan AusmanIsabella Caniggia

Abstract

Mitochondria are in a constant balance of fusing and dividing in response to cellular cues. Fusion creates healthy mitochondria, whereas fission results in removal of non-functional organelles. Changes in mitochondrial dynamics typify several human diseases. However, the contribution of mitochondrial dynamics to preeclampsia, a hypertensive disorder of pregnancy characterized by placental cell autophagy and death, remains unknown. Herein, we show that the mitochondrial dynamic balance in preeclamptic placentae is tilted toward fission (increased DRP1 expression/activation and decreased OPA1 expression). Increased phosphorylation of DRP1 (p-DRP1) in mitochondrial isolates from preeclamptic placentae and transmission electron microscopy corroborated augmented mitochondrial fragmentation in cytotrophoblast cells of PE placentae. Increased fission was accompanied by build-up of ceramides (CERs) in mitochondria from preeclamptic placentae relative to controls. Treatment of human choriocarcinoma JEG3 cells and primary isolated cytrophoblast cells with CER 16:0 enhanced mitochondrial fission. Loss- and gain-of-function experiments showed that Bcl-2 member BOK, whose expression is increased by CER, positively regulated p-DRP1/DRP1 and ...Continue Reading

References

Sep 14, 2000·Placenta·C W Redman, I L Sargent
Aug 22, 2001·Molecular Biology of the Cell·E SmirnovaA M van der Bliek
Nov 14, 2001·Laboratory Investigation; a Journal of Technical Methods and Pathology·D J MacPheeI Caniggia
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Jan 28, 2004·Cell·Nika N Danial, Stanley J Korsmeyer
Mar 5, 2004·Biochimica Et Biophysica Acta·Anna SchinzelChristoph Borner
Aug 7, 2004·Science·Takumi KoshibaDavid C Chan
Mar 19, 2005·Cell Death and Differentiation·N SoleymanlouI Caniggia
Feb 16, 2007·The Journal of Biological Chemistry·Naoko TaguchiKatsuyoshi Mihara
Jul 4, 2007·Proceedings of the National Academy of Sciences of the United States of America·Craig BrooksZheng Dong
Nov 17, 2007·Cardiovascular Research·Valentina ParraSergio Lavandero
Sep 16, 2009·The Journal of Cell Biology·Junko WakabayashiHiromi Sesaki
Dec 30, 2009·The Journal of Cell Biology·Brian HeadAlexander M van der Bliek
Dec 30, 2009·The Journal of Cell Biology·Sarah EhsesThomas Langer
Feb 18, 2010·FEBS Letters·Vidyaramanan Ganesan, Marco Colombini
Aug 19, 2010·The EMBO Journal·Olga Martins de Brito, Luca Scorrano
Apr 15, 2011·Journal of Lipids·Denny ProkschChristoph Arenz
Jun 23, 2011·PloS One·Hyunmi LeeRichard Kolesnick
Dec 21, 2011·Biochimica Et Biophysica Acta·Stefan Grimm
Feb 16, 2012·The Journal of Biological Chemistry·Stefan Strack, J Thomas Cribbs
Sep 5, 2012·Biological Chemistry·Wen-Xing Ding, Xiao-Ming Yin
Oct 17, 2012·Biochemical and Biophysical Research Communications·Kei OkatsuNoriyuki Matsuda
Jan 4, 2013·Molecular Biology of the Cell·Oliver C LosónDavid C Chan
Feb 23, 2013·Cell Death and Differentiation·N EcheverryT Kaufmann
Mar 2, 2013·American Journal of Respiratory and Critical Care Medicine·John J RyanStephen L Archer
Oct 1, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Willard W SharpStephen L Archer
Oct 12, 2013·Autophagy·Manpreet KalkatIsabella Caniggia
Dec 7, 2013·The New England Journal of Medicine·Stephen L Archer
Jan 31, 2014·Current Atherosclerosis Reports·Amanda R Vest, Leslie S Cho
Mar 13, 2014·The Journal of Cell Biology·Ruchika AnandThomas Langer
Apr 16, 2014·PloS One·Laura M WestrateJeffrey P MacKeigan
Apr 17, 2014·Trends in Neurosciences·Leslie A ScarffeTed M Dawson

❮ Previous
Next ❯

Citations

Jul 18, 2019·Proceedings of the National Academy of Sciences of the United States of America·Rahul SrivastavaHamsa Puthalakath
Aug 31, 2019·Clinical and Experimental Pharmacology & Physiology·Joshua J FisherOlivia J Holland
Apr 9, 2020·Biology·Dmitry O IvanovRuslan A Nasyrov
Aug 30, 2019·Journal of Receptor and Signal Transduction Research·Long BaoZhixiong Lin
Nov 21, 2018·Cell Death & Disease·Olivia J HollandAnthony V Perkins
Jul 28, 2020·Nutrients·Ameyalli M Rodríguez-CanoOtilia Perichart-Perera
Jun 30, 2019·Oxidative Medicine and Cellular Longevity·Samprikta MannaFergus P McCarthy
Jun 23, 2019·Cell and Tissue Research·Jacqui DetmarAndrea Jurisicova
Feb 6, 2019·International Journal of Oncology·Susana Romero-Garcia, Heriberto Prado-Garcia
Jan 3, 2019·The Journal of Clinical Investigation·Estela Area-GomezSerge Przedborski
Mar 4, 2020·Cells·Lais Brigliadori FugioAndréia Machado Leopoldino
Oct 13, 2020·Frontiers in Cell and Developmental Biology·Samara Naim, Thomas Kaufmann
Jan 12, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Esha GangulySandra T Davidge
Sep 12, 2020·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Reinaldo MarínLuis Sobrevia
Feb 2, 2021·Reproductive Sciences·Philippe VangriekenAlex H V Remels
Oct 24, 2020·Proceedings of the National Academy of Sciences of the United States of America·Estefanía LucendoMar Orzáez
May 16, 2021·Cellular Signalling·Yuliya FakhrDenise G Hemmings
Jul 3, 2021·Biomolecules·Antía CustodiaAlberto Ouro
Aug 17, 2021·Pediatric Neurology·Nina F Schor, Diana W Bianchi
Oct 5, 2021·Frontiers in Pharmacology·Hongyang ShuNing Zhou
Oct 21, 2021·The FEBS Journal·Robert E Means, Samuel G Katz

❮ Previous
Next ❯

Methods Mentioned

BETA
Transmission electron microscopy
transfection
proximity ligation assay
GTPase
cesarean sections
protein assay
electrophoresis
X-ray
Assay

Software Mentioned

ImageQuant®
GraphPad Prism
ImageJ®
Volocity

Related Concepts

Related Feeds

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.