PMID: 11310790Apr 20, 2001Paper

Ceramide inhibits cell proliferation through Akt/PKB inactivation and decreases melanin synthesis in Mel-Ab cells

Pigment Cell Research
Dong-Seok KimK C Park

Abstract

Ceramide is a bioactive sphingolipid that mediates a variety of cell functions. However, the effects of ceramide on cell growth and the melanogenesis of melanocytes are not known. In the present study, we investigated the actions of cell-permeable ceramide and its possible role in the signaling pathway of a spontaneously immortalized mouse melanocyte cell line, Mel-Ab. Our results show that C2-ceramide inhibits DNA synthesis in Mel-Ab cells and G361 human melanoma cells in a dose-dependent manner. Cell cycle analysis confirmed the inhibition of DNA synthesis by a reduction in the S phase. To investigate the ceramide signaling pathway, we studied whether C2-ceramide is able to influence extracellular signal-regulated kinase (ERK) and/or Akt/protein kinase B (PKB) activation. We demonstrated that phosphorylated Akt/PKB is decreased by C2-ceramide, whereas phosphorylated ERK was only slightly affected. Therefore, the C2-ceramide-induced inactivation of Akt/PKB may be closely related to the reduced cell proliferation of Mel-Ab cells. Furthermore, we assessed the effects of C2-ceramide on the pigmentation of Mel-Ab cells. The results obtained showed that the melanin content of cells was significantly reduced by C2-ceramide at concen...Continue Reading

Citations

Jun 29, 2002·Journal of Biosciences·Shampa MallickRanjan Bhadra
Jan 16, 2004·Archives of Pharmacal Research·Dong-Seok KimKyoung-Chan Park
Oct 2, 2002·Journal of Dermatological Science·Won Suk HanKyu Han Kim
Aug 16, 2011·Journal of Biomedical Science·Hyo-Soon JeongDong-Seok Kim
Nov 10, 2007·Journal of Oleo Science·Mikio KinoshitaMasao Ohnishi
Sep 24, 2013·Biochimica Et Biophysica Acta·Yoshikazu Uchida
Mar 1, 2012·Annals of Dermatology·Hyojin KimKyoung-Chan Park
Nov 25, 2003·Pigment Cell Research·Kazuhiko HiguchiGenji Imokawa
Mar 8, 2003·Pigment Cell Research·Stefania BrigantiMauro Picardo
Nov 6, 2007·Experimental Dermatology·Dong-Seok KimKyoung-Chan Park
Mar 10, 2006·Pigment Cell Research·Dong-Seok KimKyoung-Chan Park
May 22, 2010·The Journal of Pharmacy and Pharmacology·Dong-Seok KimKyoung-Chan Park
Jun 8, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ilona SilinsUlla Stenius
Sep 7, 2004·Free Radical Research·Shikibu MuranakaKozo Utsumi
Feb 15, 2020·Biology of Reproduction·Eleanore V O'NeilThomas E Spencer
May 7, 2020·Biotechnology and Applied Biochemistry·Jian ChenYong-Bing Cao
Apr 5, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kazuhiro KagotaniYasuhito Shimada
May 26, 2016·International Journal of Molecular Medicine·Hwa Jun ChaSungkwan An
Oct 3, 2020·International Journal of Molecular Sciences·Riad LadjohounlouClaire Rodriguez-Lafrasse
Mar 1, 2017·International Journal of Molecular Sciences·Jae Kyoung ChaeSun Yeou Kim
Apr 27, 2018·Journal of Extracellular Vesicles·Ryan C MiddletonEduardo Marbán
Jun 4, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Melissa A TranGavin P Robertson
May 30, 2002·Cellular Signalling·Dong-Seok KimKyoung-Chan Park
Jan 18, 2003·Biochimica Et Biophysica Acta·Benjamin J PettusYusuf A Hannun

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.