Ceramide inhibits the inwardly rectifying potassium current in GH(3) lactotrophs

Endocrinology
S N WuH T Chiang

Abstract

The effects of ceramide on ion currents in rat pituitary GH(3) cells were investigated. Hyperpolarization-elicited K(+) currents present in GH(3) cells were studied to determine the effect of ceramide and other related compounds on the inwardly rectifying K(+) current (I(K(IR))). Ceramide (C(2)-ceramide) suppressed the amplitude of I(K(IR)) in a concentration-dependent manner, with an IC(50) value of 5 microM. Ceramide caused a rightward shift in the midpoint for the activation curve of I(K(IR)). Pretreatment with PD-98059 (30 microM) or U-0126 (30 microM) did not prevent ceramide-mediated inhibition of I(K(IR)). However, the magnitude of ceramide-induced inhibition of I(K(IR)) was attenuated in GH(3) cells preincubated with dithiothreitol (10 microM). TNF alpha (100 ng/g) also suppressed I(K(IR)). In the inside-out configuration, application of ceramide (30 microM) to the bath slightly suppressed the activity of large conductance Ca(2+)-activated K(+) channels. Under the current clamp mode, ceramide (10 microM) increased the firing of action potentials. Cells that exhibited an irregular firing pattern were converted to those displaying a regular firing pattern after application of ceramide (10 microM). Ceramide also suppressed...Continue Reading

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Citations

Jun 23, 2010·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Stefano La RosaCarlo Capella
May 8, 2004·Molecular and Cellular Endocrinology·C L ChikA K Ho
Dec 2, 2004·Brain Research. Brain Research Reviews·Laura Colombaioni, Mercedes Garcia-Gil
Oct 19, 2006·Experimental and Molecular Pathology·Saumya PandeyDevendra K Agrawal
Oct 28, 2009·Cellular and Molecular Life Sciences : CMLS·Cia RamströmKid Törnquist
Jun 3, 2009·Apoptosis : an International Journal on Programmed Cell Death·Ying ZhangFlorian Lang
Jun 4, 2011·Expert Opinion on Therapeutic Targets·Florian LangErich Gulbins
Dec 13, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Polina S Petkova-KirovaGuy Salama

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