Ceramide reduces endothelium-dependent vasodilation by increasing superoxide production in small bovine coronary arteries

Circulation Research
D X ZhangP L Li

Abstract

Ceramide serves as a second messenger in a variety of mammalian cells. Little is known regarding the role of ceramide in the regulation of vascular endothelial function. The present study was designed to determine whether ceramide affects endothelium-dependent vasodilation in coronary arteries and to explore the mechanism of action of ceramide. In isolated and pressurized small bovine coronary arteries, cell-permeable C(2)-ceramide (10(-)(5) mol/L) markedly attenuated vasodilator responses to bradykinin and A23187 (by 40% and 60%, respectively). In the presence of K(G)-nitro-L-arginine methyl ester, ceramide produced no further inhibition on the vasodilation induced by these vasodilators. Ceramide had no effect on DETA NONOate-induced vasodilation. By use of a fluorescence NO indicator (4,5-diaminofluorescein diacetate), intracellular NO was measured in the endothelium of freshly isolated small coronary arteries. It was found that ceramide significantly inhibited bradykinin-induced NO increase within endothelial cells. However, it had no effect on the activity of arterial or endothelial NO synthase. Pretreatment of the arteries with sodium dihydroxybenzene disulfonate (Tiron, 10(-)(3) mol/L), a cell-permeable superoxide scaveng...Continue Reading

References

Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D S Bredt, S H Snyder
May 1, 1986·The American Journal of Physiology·G M Rubanyi, P M Vanhoutte
Sep 1, 1993·The American Review of Respiratory Disease·J XieS S Greenberg
Jun 20, 1997·The Journal of Biological Chemistry·A K BhuniaS Chatterjee
Aug 8, 1997·Biochemical and Biophysical Research Communications·D G JohnsR C Webb
Dec 31, 1997·The Journal of Clinical Investigation·D G Harrison
Feb 17, 1999·Journal of Molecular and Cellular Cardiology·D V DeFily
Jul 23, 1999·Annals of the New York Academy of Sciences·J Vinten-JohansenR A Guyton
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·J IgarashiT Michel
Apr 25, 2000·American Journal of Physiology. Heart and Circulatory Physiology·T ZhengB M Altura
Jun 1, 2000·Biochemistry·M Di PaolaM Lorusso
Jun 1, 2000·Biochimica Et Biophysica Acta·A HuwilerK Sandhoff

❮ Previous
Next ❯

Citations

May 15, 2002·Trends in Pharmacological Sciences·Stavros Selemidis, Thomas M Cocks
Jan 30, 2002·British Journal of Pharmacology·Peter CzyborraAngela Bischoff
Aug 7, 2002·British Journal of Pharmacology·Alberto ChiarugiSalvatore Salomone
Aug 15, 2006·Biochemistry. Biokhimii︠a︡·O M IpatovaE M Khalilov
Aug 30, 2008·American Journal of Respiratory and Critical Care Medicine·Stefan Uhlig, Erich Gulbins
Sep 12, 2008·Biological Chemistry·Heike GrassméErich Gulbins
Jun 21, 2014·Journal of Diabetes Research·M E HansenB T Bikman
Oct 30, 2007·Atherosclerosis·Jean BismuthChangyi Chen
Oct 27, 2004·British Journal of Pharmacology·Astrid E AlewijnseMartin C Michel
Mar 30, 2007·Acta Paediatrica. Supplement·Martin C MichelStephan L M Peters
May 24, 2006·Free Radical Biology & Medicine·Je-Seong Won, Inderjit Singh
Nov 19, 2005·Life Sciences·Sandrine LecourLionel H Opie
Sep 6, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Haiqiang JiangChuanhua Yang
Jan 31, 2006·Developmental Biology·Anjali K Nath, Joseph A Madri
Jun 24, 2005·American Journal of Physiology. Renal Physiology·Ningjun LiPin-Lan Li
Jan 21, 2006·Journal of Cardiovascular Pharmacology·Sandrine LecourMichael N Sack
Oct 26, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Guo ZhangPin-Lan Li
Dec 15, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Erich Gulbins, Pin Lan Li
Sep 12, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Guo ZhangPin-Lan Li
Apr 11, 2003·American Journal of Physiology. Cell Physiology·Sukhdev S BrarJohn R Hoidal
Oct 9, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Andrew Y ZhangPin-Lan Li
May 14, 2005·Endocrinology·Kathryn M GauthierWilliam B Campbell
Apr 14, 2015·Biological Chemistry·Peter L JerniganTimothy A Pritts
Dec 19, 2001·American Journal of Physiology. Heart and Circulatory Physiology·Leonard KleineRichard L Hébert
Aug 16, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Ningjun LiAi-Ping Zou
Nov 9, 2002·American Journal of Physiology. Heart and Circulatory Physiology·David X ZhangPin-Lan Li
Jan 24, 2019·International Journal of Molecular Sciences·Angel CogolludoLaura Moreno
Jan 18, 2020·Annals of Clinical and Translational Neurology·Emer R McGrathSudha Seshadri
Aug 22, 2020·Journal of Lipid and Atherosclerosis·Anna Kovilakath, L Ashley Cowart
Oct 22, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Fu-Xian YiPin-Lan Li
Oct 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·David X ZhangPin-Lan Li
Jun 5, 2003·American Journal of Physiology. Renal Physiology·Andrew Y ZhangAi-Ping Zou
Oct 31, 2014·American Journal of Physiology. Renal Physiology·Rocio Bautista-PérezMartha Franco
Jun 14, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Francis A SylvesterJulian H Lombard
Sep 20, 2019·Journal of Diabetes Research·Daniel W Nuno, Kathryn G Lamping
Feb 16, 2021·Frontiers in Endocrinology·Rebekah J NicholsonScott A Summers
Sep 14, 2002·The Journal of Laboratory and Clinical Medicine·Douglas Peterson, E Kenneth Weir

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.