PMID: 8948473Nov 13, 1996Paper

Cercospora beticola toxins. Part XVII. The role of the beticolin/Mg2+ complexes in their biological activity. Study of plasma membrane H(+)-ATPase, vacuolar H(+)-PPase, alkaline and acid phosphatases

Biochimica Et Biophysica Acta
E. GomesJ. P. Blein

Abstract

Beticolin-1 and beticolin-2, yellow toxins produced by the phytopathogenic fungus Cercospora beticola, inhibit the plasma membrane H(+)-ATPase. Firstly, since beticolins are able to form complexes with Mg2+, the role of the beticolin/Mg2+ complexes in the inhibition of the plasma membrane proton pump has been investigated. Calculations indicate that beticolins could exist under several forms, in the H(+)-ATPase assay mixture, both free or complexed with Mg2+. However, the percentage inhibition of the H(+)-ATPase activity is correlated to the concentration of one single form of beticolin, the dimeric neutral complex Mg2H2B2, which appears to be the active form involved in the H(+)-ATPase inhibition. Secondly, since previous data suggested that beticolins could also be active against other Mg2(+)-dependent enzymes, we tested beticolin-1 on the vacuolar H(+)-PPase, which requires Mg2+ as co-substrate, and on the alkaline and acid phosphatases, which do not use Mg2+ as co-substrate. Only vacuolar H(+)-PPase is sensitive to beticolin-1, which suggests that beticolins are specific to enzymes that use a complex of Mg2+ as the substrate. The same Mg2H2B2 complex which is responsible of the plasma membrane H(+)-ATPase inhibition appears...Continue Reading

References

Jan 1, 1991·Annual Review of Physiology·P W Flatman
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P AducciR Scalla
Dec 1, 1996·Plant Cell Reports·A Oostdam, L H van der Plas

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Citations

Feb 11, 2005·Planta·Nora Gutiérrez-NájeraMarina Gavilanes-Ruíz
Mar 23, 2012·Brazilian Journal of Biology = Revista Brasleira De Biologia·M Pérez-SayánsA García-García
Jul 19, 2020·Molecular Plant Pathology·Lorena I RangelMelvin D Bolton
May 24, 2012·Chemical Reviews·Kye-Simeon Masters, Stefan Bräse

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