Cerebellar Predominant Increase in mRNA Expression Levels of Sirt1 and Sirt3 Isoforms in a Transgenic Mouse Model of Huntington's Disease.

Neurochemical Research
Andras SalamonDénes Zádori

Abstract

The potential role of Sirt1 and Sirt2 subtypes of Sirtuins (class III NAD+-dependent deacetylases) in the pathogenesis of Huntington's disease (HD) has been extensively studied yielding some controversial results. However, data regarding the involvement of Sirt3 and their variants in HD are considerably limited. The aim of this study was to assess the expression pattern of Sirt1 and three Sirt3 mRNA isoforms (Sirt3-M1/2/3) in the striatum, cortex and cerebellum in respect of the effect of gender, age and the presence of the transgene using the N171-82Q transgenic mouse model of HD. Striatal, cortical and cerebellar Sirt1-Fl and Sirt3-M1/2/3 mRNA levels were measured in 8, 12 and 16 weeks old N171-82Q transgenic mice and in their wild-type littermates. Regarding the striatum and cortex, the presence of the transgene resulted in a significant increase in Sirt3-M3 and Sirt1 mRNA levels, respectively, whereas in case of the cerebellum the transgene resulted in increased expression of all the assessed subtypes and isoforms. Aging exerted minor influence on Sirt mRNA expression levels, both in transgene carriers and in their wild-type littermates, and there was no interaction between the presence of the transgene and aging. Furthermo...Continue Reading

References

Nov 1, 1985·Journal of Neuropathology and Experimental Neurology·J P VonsattelE P Richardson
Jul 1, 1984·Experimental Neurology·D V JesteJ Parisi
Apr 1, 1981·Journal of the Neurological Sciences·R A Rodda
Feb 5, 1993·Neuroscience Letters·M TakadaT Hattori
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Wenzhen DuanMark P Mattson
Jun 26, 2003·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·F SquitieriG Tortorella
Jan 28, 2004·Neurogenetics·Anton van Dellen, Anthony J Hannan
Oct 21, 2004·The Journal of Biological Chemistry·Gabriella GardianM Flint Beal
May 12, 2005·Médecine sciences : M/S·Alex J ParkerChristian Néri
Mar 30, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jim P DompierreFrédéric Saudou
Jan 29, 2008·Recent Patents on CNS Drug Discovery·Mercè PallàsAntoni Camins
Sep 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Bong-Hyun AhnToren Finkel
Jun 25, 2009·Journal of Neural Transmission·Eniko VamosPeter Klivenyi
Aug 25, 2009·Pharmacology, Biochemistry, and Behavior·Dénes ZádoriPéter Klivényi
Nov 26, 2009·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Eniko VamosPeter Klivenyi
Jun 22, 2010·Experimental Neurology·Daniel J HoM Flint Beal
Aug 3, 2010·Journal of Cellular Biochemistry·Yongjie YangQiang Tong
Jan 5, 2011·Journal of Neural Transmission·Dénes ZádoriPéter Klivényi
Jul 28, 2011·Human Molecular Genetics·Michele M MaxwellAleksey G Kazantsev
Jan 10, 2012·Nature Medicine·Albert R La Spada
Dec 4, 2012·Cell Reports·Vanita ChopraAleksey G Kazantsev
Mar 23, 2013·Biochemistry·Daniel C Liebler, Lisa J Zimmerman
Dec 25, 2013·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Samuel Frank
Aug 16, 2014·Movement Disorders : Official Journal of the Movement Disorder Society·Elin M ReesNicola Z Hobbs
Sep 17, 2014·British Journal of Clinical Pharmacology·Sigurd D SüssmuthUNKNOWN PADDINGTON Consortium
Oct 17, 2014·Cellular and Molecular Neurobiology·Rita TörökPéter Klivényi
Jul 15, 2015·Biochimica Et Biophysica Acta·Luana Naia, A Cristina Rego

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
electrophoresis
PCR

Software Mentioned

R
R Development Core Team

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.

Cell Aging

This feed focuses on cellular aging with emphasis on mitochondria, autophagy, and metabolic processes associated with aging and longevity. Here is the latest research on cell aging.

Cell Aging (Keystone)

This feed focuses on cellular aging with emphasis on the mitochondria, autophagy, and metabolic processes associated with aging and longevity. Here is the latest research on cell aging.