Cerebellar Purkinje cell loss in aging Hu-Bcl-2 transgenic mice

The Journal of Comparative Neurology
Hadi ZanjaniJean Mariani

Abstract

The number of cerebellar Purkinje cells is increased by over 40% in young transgenic mice that overexpress a human Bcl-2 transgene (Hu-Bcl-2). To determine whether the Bcl-2-mediated rescue of Purkinje cells persists through life, the numbers of Purkinje cells were estimated in 6-, 12-, 18-, and 24-month-old Hu-Bcl-2 transgenic mice and age-matched controls. In addition, the expression of four markers for Purkinje cell differentiation, calbindin (CaBP), the 67-kDa isoform of glutamic acid decarboxylase (GAD67), gamma-aminobutyric acid transaminase (GABA-T), and the NMDA-R1 receptor subtype (NMDA-NR1) was analyzed in 6-month-old Hu-Bcl-2 transgenics and controls to determine whether overexpression of Bcl-2 and rescue from naturally occurring cell death affects the normal differentiation of Purkinje cells. The estimates of Purkinje cell numbers showed that the number of Purkinje cells in the Hu-Bcl-2 transgenics declines after 6 months to approach wild-type values by 18 months. Although the exogenous human BCL-2 is still expressed in Purkinje cells at 24 months, the expression levels of human BCL-2 appear to decline significantly after 6 months, suggesting that survival of the supernumary Purkinje cells depends on the sustained o...Continue Reading

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Citations

Oct 14, 2010·The Cerebellum·Jakob JankowskiStephan Baader
Jan 7, 2005·Anatomical Science International·Robert R Buss, Ronald W Oppenheim
Jun 17, 2006·Annual Review of Neuroscience·Robert R BussRonald W Oppenheim
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Nov 17, 2020·Frontiers in Cell and Developmental Biology·Simone Macrì, Nicolas Di-Poï

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