Cerebral Apolipoprotein-D Is Hypoglycosylated Compared to Peripheral Tissues and Is Variably Expressed in Mouse and Human Brain Regions

PloS One
Hongyun LiBrett Garner

Abstract

Recent studies have shown that cerebral apoD levels increase with age and in Alzheimer's disease (AD). In addition, loss of cerebral apoD in the mouse increases sensitivity to lipid peroxidation and accelerates AD pathology. Very little data are available, however, regarding the expression of apoD protein levels in different brain regions. This is important as both brain lipid peroxidation and neurodegeneration occur in a region-specific manner. Here we addressed this using western blotting of seven different regions (olfactory bulb, hippocampus, frontal cortex, striatum, cerebellum, thalamus and brain stem) of the mouse brain. Our data indicate that compared to most brain regions, the hippocampus is deficient in apoD. In comparison to other major organs and tissues (liver, spleen, kidney, adrenal gland, heart and skeletal muscle), brain apoD was approximately 10-fold higher (corrected for total protein levels). Our analysis also revealed that brain apoD was present at a lower apparent molecular weight than tissue and plasma apoD. Utilising peptide N-glycosidase-F and neuraminidase to remove N-glycans and sialic acids, respectively, we found that N-glycan composition (but not sialylation alone) were responsible for this reducti...Continue Reading

References

Jun 1, 1995·Brain Research. Molecular Brain Research·D SéguinE Rassart
Oct 21, 1998·Neuroscience Letters·A NavarroE del Valle
Jan 20, 1999·Cellular and Molecular Life Sciences : CMLS·C Traving, R Schauer
Jun 25, 1999·Brain Research. Molecular Brain Research·L TerrisseE Rassart
Nov 4, 2000·Biochimica Et Biophysica Acta·E RassartR Milne
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·E A ThomasJ G Sutcliffe
Mar 29, 2001·Journal of Neuroscience Research·B BelloirA Savioz
Dec 12, 2001·Journal of Neurochemistry·E A ThomasJ G Sutcliffe
Dec 18, 2001·Mechanisms of Development·Diego SánchezSalvador Martínez
May 7, 2008·Proceedings of the National Academy of Sciences of the United States of America·Julien MuffatSeymour Benzer
Apr 6, 2011·Journal of Neurochemistry·Raquel Bajo-GrañerasMaria D Ganfornina
Nov 28, 2013·The FEBS Journal·Sophia Schedin-WeissLars O Tjernberg
Mar 13, 2014·Neurobiology of Aging·Sarah DassatiRüdiger Schweigreiter
Mar 20, 2014·Glycobiology·Hilary Scott, Vladislav M Panin
Sep 5, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ye ZhangJian Qian Wu
Nov 27, 2014·Frontiers in Cellular Neuroscience·Nadia García-MateoDiego Sanchez
Mar 19, 2015·Neurobiology of Aging·Hongyun LiBrett Garner
Apr 15, 2015·Experimental Gerontology·Diego SanchezMaria D Ganfornina

❮ Previous
Next ❯

Citations

Sep 6, 2019·Medicines·Patricia ReganMargaret Doherty
May 1, 2021·International Journal of Molecular Sciences·Frederik DesmaraisCatherine Mounier
Oct 26, 2021·Frontiers in Physiology·Diego Sanchez, Maria D Ganfornina

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
ELISA
glycosylation
dissection

Software Mentioned

ImageJ

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.