Cerebral endothelial derived vascular endothelial growth factor promotes the migration but not the proliferation of oligodendrocyte precursor cells in vitro.

Neuroscience Letters
Kazuhide HayakawaKen Arai

Abstract

In gray matter, cerebral endothelium is known to provide trophic support for neighboring cells such as neurons. However, signaling from cerebral endothelium to white matter cells remains to be elucidated. Here, we show that vascular endothelial growth factor (VEGF-A) secreted from cerebral endothelial cells promotes the migration but not the proliferation of oligodendrocyte precursor cells (OPCs). Cultured OPCs were obtained from newborn rat cortex, and treatment with conditioned culture media of cerebral endothelial cells increased the OPC proliferation and migration. Importantly, co-treatment with anti-neutralizing antibody for Flk-1 (VEGF-receptor2) inhibited OPC movement but did not affect OPC propagation. Western blot and flow cytometry analyses confirmed that our cultured cerebral endothelial cells produced VEGF-A and our cultured OPCs expressed Flk-1. Taken together, our current data suggest that cerebral endothelium is supportive for oligodendrocyte lineage cells and VEGF-A may participate in the endothelium-OPC cell-cell signaling. This phenomenon may be important for white matter homeostasis.

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Citations

Aug 9, 2013·Translational Stroke Research·M DzietkoD M Ferriero
Oct 18, 2013·Cellular and Molecular Life Sciences : CMLS·Nobukazu MiyamotoKen Arai
Mar 12, 2013·Cellular and Molecular Life Sciences : CMLS·Peter CarmelietRuiz de Almodovar Carmen
Apr 30, 2015·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Akihiro ShindoKen Arai
Aug 19, 2015·Biochimica Et Biophysica Acta·Kazuhide Hayakawa, Eng H Lo
Nov 10, 2016·Behavioural Brain Research·Hajime TakaseJosephine Lok
Jun 12, 2018·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Poornima VenkatMichael Chopp
Jul 6, 2014·Physiological Reviews·Leda Dimou, Magdalena Götz
Aug 15, 2019·CNS Neuroscience & Therapeutics·Minkyung Kang, Yao Yao
Jun 9, 2020·International Journal of Cancer. Journal International Du Cancer·Erik JungFrank Winkler
Jun 14, 2018·International Journal of Molecular Sciences·Ryo OhtomoKen Arai
May 11, 2021·Journal of Molecular Neuroscience : MN·Yi-Sha GuoXia Bi
Aug 28, 2021·International Journal of Molecular Sciences·Sunhong MoonYoon Kyung Choi
Oct 14, 2021·Developmental Neurobiology·Wenlong Xia, Stephen P J Fancy

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