Cerebral ischemia affects glucose transporter kinetics across rat brain microvascular endothelium: quantitative analysis by an in situ brain perfusion method
Abstract
It has been reported that cerebral ischemia induces a dissociation between cerebral blood flow and blood-brain barrier glucose transport, but mechanisms of the dissociation are not yet clearly understood. Recent immunohistochemical studies reveal discrepancies of the results between physiologic and immunochemical studies. The purpose of this study was to quantify changes of the blood-brain barrier glucose transporter kinetics following cerebral ischemia by an in situ brain perfusion technique. Fifty-six adult male Sprague-Dawley rats were divided into control and ischemia groups, and four-vessel occlusion was done as an ischemic insult. To obtain regional capillary permeability surface area products of glucose and regional perfusion fluid flow rates, the perfusion fluid (HCO3-buffered saline) was dually labeled with [14C]-2-Deoxyglucose and [3H]-Diazepam, and the brain was perfused at a constant rate via the external carotid artery. After sampling tissues from three regions (frontal, frontoparietal lobe, and caudoputamen), dual scintillation counting was performed. From the results, we determined kinetic parameters, including Vmax, Km, and Kd as described in the Michaelis-Menten equation, by weighted nonlinear least squares met...Continue Reading
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Brain Ischemia
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