Cerebrospinal fluid from amyotrophic lateral sclerosis has no effect on intracellular free calcium in cultured cortical neurons

Molecular and Chemical Neuropathology
O GredalM Nielsen

Abstract

The data from the literature regarding the presence of a neurotoxic factor in amyotrophic lateral sclerosis (ALS) plasma or cerebrospinal fluid (CSF) remain controversial. As a new approach to this question, we have studied the effect of CSF from ALS patients on the temporal dynamics of the intracellular free calcium concentration ([Ca2+]i) of murine cortical neurons in cultures using Fura-2 fluorescence videomicroscopy and single-cell imaging. CSF from seven ALS patients and controls was added at dilutions up to 20% to cortical neuronal cultures. The in vitro inhibition of CSF on [3H]kainic acid binding showed that the CSF did not contain any substances other than glutamate itself in larger amounts. At the concentrations used, the CSF did not have any effect on [Ca2+]i or on the neuronal responsiveness as defined by the ability of the cells to respond with a transient increase in [Ca2+]i to depolarization induced by KCl. The disturbance of the intracellular calcium homeostasis is one of the key mechanisms of action of excitotoxic compounds mediating delayed neuronal cell death by stimulation of glutamate receptor subtypes. In this study, CSF from ALS patients did not induce immediate rises in [Ca2+]i or disturbances of the int...Continue Reading

References

Apr 1, 1978·Annals of Neurology·B M PattenM T Felmus
Aug 11, 1975·Acta Neuropathologica·J LivesonM B Bornstein
May 28, 1992·The New England Journal of Medicine·J D RothsteinR W Kuncl
Mar 1, 1992·Acta Neurologica Scandinavica·J Martí-FàbregasI Illa
Aug 1, 1991·Annals of Neurology·J D RothsteinD B Drachman
Jan 1, 1990·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·A Frandsen, A Schousboe
Jul 1, 1990·Annals of Neurology·J D RothsteinJ T Coyle
Nov 1, 1987·Annals of Neurology·A Plaitakis, J T Caroscio
Apr 1, 1974·Archives of Neurology·M S HorwichP B Chauvin
Apr 14, 1994·Neuroreport·P CouratierJ Hugon
Jan 1, 1994·Journal of Neurology, Neurosurgery, and Psychiatry·O BlinG Serratice
Jul 1, 1993·Acta Neurologica Scandinavica·W CamuM Baldy-Moulinier
Apr 1, 1993·Journal of Neurochemistry·A Frandsen, A Schousboe

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Citations

May 8, 2009·Molecular Neurodegeneration·Philippe MarambaudValérie Vingtdeux
Jul 29, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hoau-Yan WangM Felice Ghilardi
Mar 26, 2002·Brain : a Journal of Neurology·Tiina M TikkaJari Koistinaho
Aug 27, 2010·Neurología : publicación oficial de la Sociedad Española de Neurología·J Matías-GuiuA Guerrero
Mar 4, 2011·Amyotrophic Lateral Sclerosis : Official Publication of the World Federation of Neurology Research Group on Motor Neuron Diseases·Siân C BarberPamela J Shaw
Apr 6, 2021·Frontiers in Molecular Neuroscience·Koy Chong Ng Kee KwongArpan R Mehta

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