Cerebrospinal fluid of newborn infants contains a deglycosylated form of the intermediate filament nestin

Pediatric Research
G GrigelionienéH Lagercrantz

Abstract

Nestin is an intermediate filament protein found in CNS progenitor cells. Nestin reappears in CNS tumor cells and reactive astrocytes after CNS injury. In this study we investigated whether nestin could be detected in the cerebrospinal fluid (CSF) of newborn infants and whether expression levels change with gestational age (GA) and/or brain injury. Using Western blot analysis, we examined the expression of nestin in the CSF of newborn infants (GA 25-42 wk) with asphyxia (n = 14), periventricular leukomalacia and peri(intra)ventricular hemorrhage (n = 7), and in a control group (n = 11). Protein extract from the periventricular brain tissue of a 1-wk-old infant was also analyzed. Nestin was detected in all the CSF samples and in the protein extract from the periventricular brain tissue. Although the CSF levels of nestin expression did not change with increasing GA, the asphyxia group had significantly lower levels of nestin in the CSF. An unexpected finding was that brain-derived nestin had an apparent molecular mass of approximately 240 kD, whereas all analyzed CSF samples contained two nestin-immunoreactive proteins at 200 and 220 kD. Experimental deglycosylation of the 240-kD form reduced the molecular mass to 220 kD, indicat...Continue Reading

References

Aug 1, 1988·Neuron·K FrederiksenR McKay
Sep 30, 1985·Biochemical and Biophysical Research Communications·F NybergL Terenius
Feb 1, 1994·Current Opinion in Cell Biology·S Heins, U Aebi
May 20, 1994·Science·C Lois, A Alvarez-Buylla
Aug 1, 1993·Differentiation; Research in Biological Diversity·P JinT Sejersen
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C Lois, A Alvarez-Buylla
Apr 1, 1995·Neurobiology of Disease·R C LinO Brüstle

❮ Previous
Next ❯

Citations

Jan 29, 2011·World Journal of Gastroenterology : WJG·Toshiyuki IshiwataZenya Naito
Jan 5, 2008·Neurological Research·Axel Petzold
Apr 5, 2014·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Michael SkwirbaVeronika Grau
Jan 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Per M AlmqvistGlenda Hendson
May 20, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Tino KleinPalle Serup
Apr 20, 2011·Journal of Cell Science·Claire L HyderJohn E Eriksson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.