Cerebrovascular endothelial cells form transient Notch-dependent cystic structures in zebrafish.

EMBO Reports
E. KuglerTimothy Ja Chico

Abstract

We identify a novel endothelial membrane behaviour in transgenic zebrafish. Cerebral blood vessels extrude large transient spherical structures that persist for an average of 23 min before regressing into the parent vessel. We term these structures "kugeln", after the German for sphere. Kugeln are only observed arising from the cerebral vessels and are present as late as 28 days post fertilization. Kugeln do not communicate with the vessel lumen and can form in the absence of blood flow. They contain little or no cytoplasm, but the majority are highly positive for nitric oxide reactivity. Kugeln do not interact with brain lymphatic endothelial cells (BLECs) and can form in their absence, nor do they perform a scavenging role or interact with macrophages. Inhibition of actin polymerization, Myosin II, or Notch signalling reduces kugel formation, while inhibition of VEGF or Wnt dysregulation (either inhibition or activation) increases kugel formation. Kugeln represent a novel Notch-dependent NO-containing endothelial organelle restricted to the cerebral vessels, of currently unknown function.

References

Nov 1, 1995·Nature Medicine·B M WeinsteinM C Fishman
Dec 9, 1996·FEBS Letters·S DimmelerA M Zeiher
Nov 11, 1999·Angewandte Chemie·H KojimaT Nagano
Mar 11, 2000·The Journal of Clinical Investigation·A JoutelE Tournier-Lasserve
Jun 15, 2000·Nature Cell Biology·W M MortonP J McLaughlin
Apr 23, 2002·Nature Genetics·Amy J SehnertDidier Y R Stainier
Aug 9, 2002·Developmental Biology·Nathan D Lawson, Brant M Weinstein
Oct 9, 2002·The Journal of Biological Chemistry·Xin ZhangJonathan V Sweedler
Jun 11, 2003·American Heart Journal·Leon Bernal-MizrachiYeon S Ahn
Jun 18, 2003·The Journal of Cell Biology·Holger GerhardtChrister Betsholtz
Jun 19, 2004·The Journal of Biological Chemistry·Mihály KovácsJames R Sellers
Oct 26, 2006·Journal of the American Chemical Society·Rebecca NotmanJamshed Anwar
Jan 30, 2007·Nature·Arndt F Siekmann, Nathan D Lawson
Jul 21, 2007·Free Radical Biology & Medicine·Sandrine LepillerJohanna Chluba
Jul 28, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Caroline GrayTimothy J A Chico
Oct 9, 2007·Biophysical Journal·Guillaume T CharrasL Mahadevan
Mar 19, 2008·Genes & Development·Neil C ChiDidier Y R Stainier
Mar 29, 2008·Cell Stem Cell·Richard Mark WhiteLeonard I Zon
Jun 17, 2008·Trends in Cardiovascular Medicine·Timothy J A ChicoDavid C Crossman
Aug 5, 2008·Current Drug Targets·Nancy L Parmalee, Jan Kitajewski
Mar 17, 2009·Nature Genetics·Benjamin M HoganStefan Schulte-Merker
Apr 7, 2009·Bioinformatics·Stephan PreibischPavel Tomancak
Sep 18, 2009·Nature·Shih-Min A HuangFeng Cong
Oct 31, 2009·PLoS Computational Biology·Katie BentleyPaul A Bates
May 15, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Ilse GeudensMieke Dewerchin
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jing DuHusseini K Manji
Apr 13, 2011·International Review of Cell and Molecular Biology·George E DavisWonshill Koh
Jan 10, 2012·Methods in Molecular Biology·Christopher E SchmittSuk-Won Jin
May 4, 2012·Cold Spring Harbor Perspectives in Medicine·Aniket V GoreBrant M Weinstein
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona

❮ Previous
Next ❯

Citations

Aug 15, 2020·Frontiers in Physiology·Kazuhide S Okuda, Benjamin M Hogan
Jan 5, 2021·The FEBS Journal·Emre YaksiNathalie Jurisch-Yaksi
Mar 2, 2021·Frontiers in Physiology·Ankan GuptaRamani Ramchandran
Mar 11, 2021·Cellular and Molecular Life Sciences : CMLS·Timothy J A Chico, Elisabeth C Kugler
Mar 15, 2021·Current Opinion in Hematology·Claudia Jasmin Rödel, Salim Abdelilah-Seyfried
Apr 1, 2021·British Journal of Pharmacology·George BowleyJovana Serbanovic-Canic

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.