PMID: 2484050Nov 1, 1989Paper

Cerium-based demonstration of phosphatase activity in plastic-embedded sections: a comparison with conventional methods

Stain Technology
H van GoorM J Hardonk

Abstract

Cerium-based methods have been used for the demonstration of several phosphatases at alkaline, acid and neutral pH in low temperature acetone-fixed, plastic-embedded sections. At alkaline pH calcium is used as capturing agent and the precipitated calcium phosphate converted to cerium phosphate. At neutral and acid pH cerium is used directly as capturing agent. Cerium phosphate is subsequently visualized using the H2O2-DAB method. A comparison has been made with conventional calcium-cobalt and lead methods. It appeared that calcium-cobalt methods are more susceptible to improvement than lead methods.

References

Mar 1, 1989·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H van GoorM J Hardonk
Jan 1, 1989·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T P DawsonB Kaissling
Feb 1, 1989·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P O GerritsM J Hardonk
Feb 1, 1986·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S Angermüller, H D Fahimi
May 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M E BeardE Holtzman
Dec 1, 1972·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A E AshfordM E McCully
Aug 1, 1967·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·N O JacobsonA C Thomsen
Oct 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J M Robinson, M J Karnovsky
Oct 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J M Robinson, M J Karnovsky
Sep 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G Arnold, E Holtzman
May 1, 1955·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H A PADYKULA, E HERMAN

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