Cerium dioxide nanoparticles did not alter the functional and morphologic characteristics of ram sperm during short-term exposure

Theriogenology
Laura FalchiSergio Ledda

Abstract

The aim of the study was to investigate the interaction and the short-term effects of increasing doses of cerium dioxide nanoparticles (CeO2 NPs) on ram spermatozoa, stored at 4 °C for up to 24 hours, on the main functional and kinematic parameters. Spermatozoa were incubated with 0, 22, 44, and 220 μg/mL of CeO2 NPs at 4 °C and submitted at 0, 2, and 24 hours to the following analyses: (1) intracellular uptake of CeO2 NPs by the spermatozoa; (2) kinematic parameters; (3) acrosome and membrane integrity; (4) integrity of DNA; (5) mitochondrial activity; (6) ROS production. The results indicated that the exposure of spermatozoa to increasing doses of nanoceria was well tolerated. No intracellular uptake of NPs by the cells was observed and both kinematic parameters and status of the membranes were not affected by the incubation with NPs (P > 0.05). Moreover, no influence on the redox status of spermatozoa and on the levels of fragmentation of DNA was reported among groups at any time (P > 0.05). The data collected provide new information about the impact of CeO2 NPs on the male gamete in large animal model and could open future perspectives about their biomedical use in the assisted reproductive techniques.

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Citations

Mar 8, 2018·Reproductive Biology and Endocrinology : RB&E·Laura FalchiSergio Ledda
Mar 22, 2019·Journal of Nanobiotechnology·Fenju QinJian Tong
Dec 20, 2018·International Journal of Veterinary Science and Medicine·Laura FalchiWaleed F A Marei
Mar 17, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ali A MohammedJulian R Jones
Feb 19, 2019·International Journal of Preventive Medicine·Maedeh HamzehFereshteh Talebpour Amiri
Aug 16, 2019·Biological Trace Element Research·Afsaneh NematiMohammadreza Gholami
May 18, 2020·Animals : an Open Access Journal From MDPI·Nesrein M Hashem, Antonio Gonzalez-Bulnes
Aug 10, 2020·Free Radical Biology & Medicine·F Sánchez-RubioM R Fernández-Santos
Jun 18, 2019·Theriogenology·Jean M FeugangPeter L Ryan
Mar 20, 2021·The Science of the Total Environment·Maingredy Rodrigues SouzaThiago Lopes Rocha
May 1, 2021·Animals : an Open Access Journal From MDPI·Ioannis A TsakmakidisConstantin M Boscos

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