Cervical cancer detection by DNA methylation analysis in urine

Scientific Reports
Barbara C SnoekRenske D M Steenbergen

Abstract

Urine samples provide a potential alternative to physician-taken or self-collected cervical samples for cervical screening. Screening by primary hrHPV testing requires additional risk assessment (so-called triage) of hrHPV-positive women. Molecular markers, such as DNA methylation, have proven most valuable for triage when applied to cervical specimens. This study was set out to compare hrHPV and DNA methylation results in paired urine and cervical scrapes, and to evaluate the feasibility of DNA methylation analysis in urine to detect cervical cancer. Urine samples (n = 41; native and sediment) and paired cervical scrapes (n = 38) from cervical cancer patients, and urine from 44 female controls, were tested for hrHPV and 6 methylation markers. Results on native urine and sediment were highly comparable. A strong agreement was found between hrHPV testing on urine and scrapes (kappa = 0.79). Also, methylation levels in urine were moderately to strongly correlated to those detected in scrapes (r = 0.508-0.717). All markers were significantly increased in urine from cervical cancer patients compared to controls and showed a good discriminatory power for cervical cancer (AUC = 0.744-0.887). Our results show a good agreement of urine...Continue Reading

References

Aug 26, 2000·The Pediatric Infectious Disease Journal·D L JacobsonK V Shah
May 4, 2002·Journal of Women's Health & Gender-based Medicine·Ilana G DzubaJorge Salmerón
Aug 31, 2002·Journal of Clinical Microbiology·Joeli A BrinkmanMichael E Hagensee
Jul 15, 2003·Journal of Medical Virology·Grazyna A StanczukAnna-Lise Williamson
Apr 6, 2006·International Journal of Cancer. Journal International Du Cancer·Jack CuzickThomas Iftner
Aug 26, 2006·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Amita GuptaBhudev C Das
Dec 30, 2006·Journal of Lower Genital Tract Disease·Francesc AlamedaSergi Serrano
Jan 11, 2007·Journal of Forensic Sciences·Donald J JohnsonKatherine A Roberts
Jun 6, 2007·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Qinghua FengNancy B Kiviat
Sep 17, 2009·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·A SehgalV Singh
Mar 19, 2011·BMC Bioinformatics·Xavier RobinMarkus Müller
Aug 6, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A VorstersP Van Damme
Sep 15, 2011·Journal of Medical Virology·Kate CuschieriHeather Cubie
Oct 26, 2012·Journal of Medical Virology·Elisabetta TanziGiovanna Orlando
Dec 4, 2013·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·Keimari MendezElizabeth R Unger
Jan 22, 2014·Annals of Oncology : Official Journal of the European Society for Medical Oncology·M G DijkstraC J L M Meijer
Jun 12, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A VorstersP Van Damme
Sep 16, 2014·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayFreddie Bray
Sep 19, 2014·BMJ : British Medical Journal·Henry C Kitchener, Gemma L Owens
Nov 30, 2014·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Kirsten RozemeijerMarjolein van Ballegooijen
Feb 19, 2015·Nursing for Women's Health·Holly B Fontenot
Dec 15, 2015·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·V SenkomagoJ S Smith
Jan 26, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Wina VerlaatRenske D M Steenbergen

❮ Previous
Next ❯

Citations

Nov 5, 2020·Clinical Epigenetics·Rianne van den HelderRenske D M Steenbergen
Jan 29, 2021·Scientific Reports·S BachR D M Steenbergen
Apr 14, 2021·Scientific Reports·Severien Van KeerAlex Vorsters
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Laura TéblickAlex Vorsters
Jul 29, 2021·Clinical Oncology : a Journal of the Royal College of Radiologists·J C Davies-OliveiraE J Crosbie
Sep 26, 2021·International Journal of Cancer. Journal International Du Cancer·Cristiana BanilaBelinda Nedjai
Nov 13, 2021·Experimental and Therapeutic Medicine·Adrian AlbulescuAnca Botezatu

❮ Previous
Next ❯

Methods Mentioned

BETA
genotyping
PCR
urine sediment
urine collection

Software Mentioned

pROC
R

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.