Cervical intraepithelial neoplasia: prognosis by combined LOH analysis of multiple loci

Gynecologic Oncology
Amina ELhamidiMing-Qing Du

Abstract

Cervical intraepithelial neoplasias (CIN) show markedly variable clinical behavior. Clinically, it is important to distinguish CIN lesions with different behaviors and identify those likely to persist and progress. The purpose of this study is to explore whether CIN lesions with different clinical behaviors can be stratified by analysis of loss of heterozygosity (LOH) at multiple loci. One hundred sixty-four cases of CIN (54 CIN1, 59 CIN2 and 51 CIN3) were screened for LOH at 12 microsatellite markers including 10 from 3p14, 3p21-22, 6p21 and 11q23. LOH was correlated with clinical follow-up data and high-risk HPV infection. In a pilot study of 71 cases of CIN, screening of 12 microsatellite markers identified four (D3S1300, D3S1260, D11S35, and D11S528) at which LOH was significantly associated with disease persistence/progression. These four markers were further investigated in a larger cohort, which brought the total number of cases examined to 164. Combined analysis of LOH at the above four loci permitted the identification of 22-47% of CIN lesions depending on the histological grade, which showed disease persistence/progression. LOH at these loci was significantly associated with HPV16 infection. Bioinformatic analysis ide...Continue Reading

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Citations

May 17, 2007·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Y-W ChoiW S Ahn
Sep 10, 2013·The American Journal of Pathology·Young A YooSang-Hyuk Chung
Aug 11, 2007·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Micaela PoetschChris Protzel
Feb 6, 2010·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Young Sam ChoiHo Sun Choi

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