Cervical, mandibular, and parotid lymph nodes of dogs naturally infected with Leishmania infantum: a histopathologic and immunohistochemistry study and its correlation with facial skin lesions

Veterinary Pathology
M M S CostaWashington Luiz Tafuri

Abstract

The parasite load in cervical, mandibular, and parotid lymph nodes and in the skin of the nose and the pinna from dogs infected with Leishmania infantum were investigated by histologic and immunohistochemical studies. Twenty-two infected dogs with and without signs of infection were examined to demonstrate correlation of signs with parasite load and the correlation of facial skin lesions with parasites in regional lymph nodes. Chronic inflammation of the skin was demonstrated in infected dogs that had no gross skin lesions, confirming that normal-appearing skin can harbor the parasite, likely playing a role in transmission. Dogs with facial skin lesions showed a higher parasite load in parotid lymph nodes than dogs without lesions of the facial skin, based on Leishman-Donovan unit analysis. Based on immunohistochemical analysis, parasite load in parotid and cervical nodes was correlated with that of skin of the nose and pinna, as was the parasite load in mandibular lymph nodes and skin of the external nose. We demonstrated a logical involvement of the lymphatic vessels and their specific anatomic draining sites.

References

Dec 1, 1993·Veterinary Parasitology·A Martínez-MorenoS Hernández-Rodríguez
Dec 25, 2003·Journal of Comparative Pathology·L Solano-GallegoL Ferrer
Jul 1, 2004·Comparative Immunology, Microbiology and Infectious Diseases·P Desjeux
Aug 11, 2004·Acta Tropica·Wanderson Geraldo LimaWagner Luiz Tafuri
Sep 8, 2004·Journal of Immunological Methods·Wagner Luiz TafuriWashington Luiz Tafuri

❮ Previous
Next ❯

Citations

Sep 22, 2009·International Journal of Experimental Pathology·Ferdinan Almeida MeloWagner Luiz Tafuri
Feb 20, 2013·International Journal of Experimental Pathology·Lucelia C SilvaWagner L Tafuri
Nov 3, 2016·Veterinary Clinical Pathology·Saverio PaltrinieriEric Zini
Mar 24, 2016·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Francine Maria de França SilvaLeucio Câmara Alves

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
light microscopy

Related Concepts