Cervical tissue engineering using silk scaffolds and human cervical cells.

Tissue Engineering. Part a
Michael HouseDavid L Kaplan

Abstract

Spontaneous preterm birth is a frequent complication of pregnancy and a common cause of morbidity in childhood. Obstetricians suspect abnormalities of the cervix are implicated in a significant number of preterm births. The cervix is composed of fibrous connective tissue and undergoes significant remodeling in preparation for birth. We hypothesized that a tissue engineering strategy could be used to develop three-dimensional cervical-like tissue constructs that would be suitable for investigating cervical remodeling. Cervical cells were isolated from two premenopausal women undergoing hysterectomy for a benign gynecological condition, and the cells were seeded on porous silk scaffolds in the presence or absence of dynamic culture and with 10% or 20% serum. Morphological, biochemical, and mechanical properties were measured during the 8-week culture period. Cervical cells proliferated in three-dimensions and synthesized an extracellular matrix with biochemical constituents and morphology similar to native tissue. Compared to static culture, dynamic culture was associated with significantly increased collagen deposition (p < 0.05), sulfated glycosaminoglycan synthesis (p < 0.05), and mechanical stiffness (p < 0.05). Serum concent...Continue Reading

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Citations

Feb 8, 2011·Annals of Biomedical Engineering·Jonathan A KlugeDavid L Kaplan
Oct 1, 2011·Nature Protocols·Danielle N RockwoodDavid L Kaplan
Sep 17, 2011·Tissue Engineering. Part a·Michael HouseDavid L Kaplan
Feb 5, 2013·Tissue Engineering. Part C, Methods·Evangelia BellasDavid L Kaplan
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Methods Mentioned

BETA
hysterectomy
hysterectomies
biopsy
Assay
biopsies

Software Mentioned

TestXpert
Leica Confocal
ImageJ

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