Cervicovaginal microbiome dysbiosis is associated with proteome changes related to alterations of the cervicovaginal mucosal barrier

Mucosal Immunology
Hanneke BorgdorffJanneke H H M van de Wijgert

Abstract

Vaginal microbiome (VMB) dysbiosis is associated with increased acquisition of HIV. Cervicovaginal inflammation and other changes to the mucosal barrier are thought to have important roles but human data are scarce. We compared the human cervicovaginal proteome by mass spectrometry of 50 Rwandan female sex workers who had previously been clustered into four VMB groups using a 16S phylogenetic microarray; in order of increasing bacterial diversity: Lactobacillus crispatus-dominated VMB (group 1), Lactobacillus iners-dominated VMB (group 2), moderate dysbiosis (group 3), and severe dysbiosis (group 4). We compared relative protein abundances among these VMB groups using targeted (abundance of pre-defined mucosal barrier proteins) and untargeted (differentially abundant proteins among all human proteins identified) approaches. With increasing bacterial diversity, we found: mucus alterations (increasing mucin 5B and 5AC), cytoskeleton alterations (increasing actin-organizing proteins; decreasing keratins and cornified envelope proteins), increasing lactate dehydrogenase A/B as markers of cell death, increasing proteolytic activity (increasing proteasome core complex proteins/proteases; decreasing antiproteases), altered antimicrobi...Continue Reading

References

Jan 1, 1985·Statistics in Medicine·J Cuzick
Jan 1, 1996·Annual Review of Biochemistry·O CouxA L Goldberg
Apr 1, 1997·Biology of Reproduction·I K GipsonJ A Hill
Aug 13, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Selvi B WilliamsThomas C VanCott
Jan 22, 2004·Current Opinion in Immunology·Peter Michael Kloetzel, Ferry Ossendorp
Aug 5, 2004·Obstetrics and Gynecology·Mark A KlebanoffWilliam W Andrews
Jan 13, 2006·Journal of Reproductive Immunology·P YasodharaT Prasanna Krishna
Jun 6, 2006·Journal of the American Society for Mass Spectrometry·Alexander MakarovStevan Horning
Aug 29, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Missag H Parseghian, Keith A Luhrs
Sep 22, 2006·Infection and Immunity·Erika V ValoreTomas Ganz
Mar 22, 2007·Journal of Proteome Research·Leonardo PereiraSrinivasa R Nagalla
Dec 15, 2007·American Journal of Respiratory Cell and Molecular Biology·Keiko UenoK Chul Kim
Apr 10, 2008·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Natalia NikolaitchoukInger Mattsby-Baltzer
May 8, 2008·Histochemistry and Cell Biology·Roland MollLutz Langbein
Jul 8, 2008·Biochimica Et Biophysica Acta·Stephan U Sixt, Burkhardt Dahlmann
Nov 27, 2008·Bioinformatics·Seth CarbonUNKNOWN Web Presence Working Group
Apr 21, 2009·Proteome Science·Geert ZegelsXaveer W M Van Ostade
Feb 4, 2010·The Journal of Biological Chemistry·Sonia S Y TeohPhillip I Bird
May 19, 2010·American Journal of Obstetrics and Gynecology·Jane HittiSrinivasa R Nagalla
Jun 29, 2010·The American Journal of Pathology·Andrei I IvanovAsma Nusrat
Aug 4, 2010·American Journal of Reproductive Immunology : AJRI·Andrea R Thurman, Gustavo F Doncel
Jan 29, 2011·American Journal of Obstetrics and Gynecology·Joke A M DolsMathilde E Boon
Dec 3, 2011·The Journal of Clinical Investigation·Rebecca M Brotman
Jan 19, 2012·Sexually Transmitted Diseases·Sarah L BraunsteinJanneke H H M van de Wijgert
Jul 10, 2012·Protein & Cell·Karine R Mayilyan
Nov 28, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Stella LiongHarry M Georgiou
Jun 27, 2013·The Journal of Antimicrobial Chemotherapy·Federica CrucianiBeatrice Vitali
Dec 7, 2013·Journal of Cellular and Molecular Medicine·Isabel BochmannBurkhardt Dahlmann
Apr 15, 2014·Nature Biotechnology·Juan A VizcaínoHenning Hermjakob
May 8, 2014·Nature Reviews. Microbiology·Hiroshi AshidaChihiro Sasakawa
May 17, 2014·American Journal of Reproductive Immunology : AJRI·Caroline Mitchell, Jeanne Marrazzo

❮ Previous
Next ❯

Citations

Nov 9, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Vineet JoagRupert Kaul
Jan 15, 2019·AIDS Research and Human Retroviruses·Lyle R McKinnonGilda Tachedjian
Jan 9, 2018·Endocrine Reviews·Janet P HapgoodZdenek Hel
Aug 31, 2019·Journal of the International AIDS Society·Ruth MwatelahSalim S Abdool Karim
Oct 2, 2019·BJOG : an International Journal of Obstetrics and Gynaecology·M Al-MemarD A MacIntyre
Jul 18, 2020·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Travis T SimsSurbhi Grover
Aug 28, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Charlotte van der VeerRobin van Houdt
Nov 28, 2017·Applied Microbiology and Biotechnology·Gregor Reid
Feb 28, 2018·Frontiers in Immunology·Helen T GrovesJohn S Tregoning
Feb 5, 2019·Microbiology Resource Announcements·Elias McCombJacques Ravel
Jun 30, 2019·Frontiers in Cellular and Infection Microbiology·Marie-Thérèse NugeyreElisabeth Menu
Sep 2, 2020·Wellcome Open Research·Andrea LiaPietro Roversi
Sep 22, 2017·Scientific Reports·Vicky JespersJanneke H H M van de Wijgert
May 24, 2018·American Journal of Reproductive Immunology : AJRI·Alicia R BerardAdam D Burgener
Apr 2, 2020·Frontiers in Immunology·Salwan Al-NasiryBoris W Kramer
May 1, 2020·International Journal of Oral Science·Jin XiaoSteven R Gill
Jul 6, 2016·Frontiers in Microbiology·Sean M WinkleMelissa M Herbst-Kralovetz
Feb 10, 2018·AIDS·McKenna C Eastment, R Scott McClelland
May 1, 2018·American Journal of Reproductive Immunology : AJRI·Frideborg BradleyAdam D Burgener
Aug 30, 2018·Disease Models & Mechanisms·Jocelyn M WesselsCharu Kaushic
May 7, 2020·Microbiome·Kenzie D BirseGrace Aldrovandi
Oct 8, 2017·Virology Journal·Anwar Suleman MallCorena de Beer
Aug 31, 2017·Scientific Reports·Anita MitraMaria Kyrgiou
Sep 26, 2019·Frontiers in Medicine·Keli HočevarBorut Peterlin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Actin-binding Proteins

Actin-binding proteins are a component of the actin cytoskeleton that play essential roles in cellular functions such as regulation of actin polymerization, maintenance of cell polarity, gene expression regulation, cell motility and many more functions. Discover the latest research on actin-binding proteins here.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.