DOI: 10.1101/451237Oct 24, 2018Paper

CFP-1 interacts with HDAC1/2 complexes in C. elegans development

BioRxiv : the Preprint Server for Biology
Bharat PokhrelJonathan Joseph Biro


Background: CFP-1 (CXXC finger binding protein 1) is an evolutionarily conserved protein that binds to non-methylated CpG-rich promoters in humans and C. elegans. This conserved epigenetic regulator is a part of the COMPASS complex that contains the H3K4me3 methyltransferase SET1 in mammals and SET-2 in C. elegans. Previous studies have indicated the importance of cfp-1 in embryonic stem cell differentiation and cell fate specification. However, neither the function nor the mechanism of action of cfp-1 is well understood at the organismal level. Here we have used cfp-1(tm6369) and set-2(bn129) C. elegans mutant to investigate the function of CFP-1 in gene induction and development. Results: We have characterised C. elegans COMPASS mutants cfp-1(tm6369) and set-2(bn129) mutants and found that both cfp-1 and set-2 play an important role in the regulation of fertility and development of the organism. Furthermore, we found that both cfp-1 and set-2 are required for H3K4 trimethylation and play a repressive role in the expression of heat shock and salt-inducible genes. Interestingly, we found that cfp-1 but not set-2 genetically interacts with Histone Deacetylase (HDAC1/2) complexes to regulate fertility, suggesting a function of CF...Continue Reading

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