DOI: 10.1101/451237Oct 24, 2018Paper

CFP-1 interacts with HDAC1/2 complexes in C. elegans development

BioRxiv : the Preprint Server for Biology
Bharat PokhrelJonathan Joseph Biro

Abstract

Background: CFP-1 (CXXC finger binding protein 1) is an evolutionarily conserved protein that binds to non-methylated CpG-rich promoters in humans and C. elegans. This conserved epigenetic regulator is a part of the COMPASS complex that contains the H3K4me3 methyltransferase SET1 in mammals and SET-2 in C. elegans. Previous studies have indicated the importance of cfp-1 in embryonic stem cell differentiation and cell fate specification. However, neither the function nor the mechanism of action of cfp-1 is well understood at the organismal level. Here we have used cfp-1(tm6369) and set-2(bn129) C. elegans mutant to investigate the function of CFP-1 in gene induction and development. Results: We have characterised C. elegans COMPASS mutants cfp-1(tm6369) and set-2(bn129) mutants and found that both cfp-1 and set-2 play an important role in the regulation of fertility and development of the organism. Furthermore, we found that both cfp-1 and set-2 are required for H3K4 trimethylation and play a repressive role in the expression of heat shock and salt-inducible genes. Interestingly, we found that cfp-1 but not set-2 genetically interacts with Histone Deacetylase (HDAC1/2) complexes to regulate fertility, suggesting a function of CF...Continue Reading

Related Concepts

Related Feeds

Cell Fate Specification

Cell fate specification is determined by complex signal transduction pathways including Notch and the Sonic hedgehog signaling pathway. Here is the latest research on regulation of cell fate determination.

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.