CFSE flow cytometric quantification of lymphocytic proliferation in extracorporeal photopheresis: use for quality control

Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis
Bertrand EvrardArlette Tridon

Abstract

Quality control is essential to validate extracorporeal photopheresis (ECP) processes. There is just one protocol based on PHA-induced proliferation. Since it involves the use of radioactive thymidine, we developed another technique using CFSE labeling. We compared the two tests in a paired series including 18 procedures. The thymidine test was valid. Once proliferation was obtained (10 patients out of 13), the CFSE test was in close agreement with it. In particular, two cases of residual proliferation after ECP were simultaneously detected by both techniques. Only the CFSE test allows targeted analysis of lymphocytes, thus identifying a surviving lymphocytic sub-population.

References

May 2, 1994·Journal of Immunological Methods·A B Lyons, C R Parish
Jan 24, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Luis MarínAna M García-Alonso
Mar 7, 2003·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·F Schooneman
Jul 25, 2003·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·F Heshmati

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Citations

Jun 9, 2015·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Katia ChieregatoGiuseppe Astori
Sep 13, 2014·Journal of Clinical Apheresis·Katherine RadwanskiKyungyoon Min
Mar 25, 2014·Dose-response : a Publication of International Hormesis Society·Rafael de Freitas E SilvaAdemir Amaral

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