PMID: 7528783Sep 1, 1994Paper

CFTR displays voltage dependence and two gating modes during stimulation

The Journal of General Physiology
H Fischer, T E Machen

Abstract

The patch-clamp technique in conjunction with current noise analysis was employed to clarify the events underlying the regulation of the CFTR (cystic fibrosis transmembrane conductance regulator) during cAMP-dependent stimulation. 3T3 fibroblast cells expressing the CFTR were stimulated in cell-attached mode with forskolin. The number (N) of activated channels per patch ranged from 1 to approximately 100. In true single-channel recordings, CFTR's gating was best described by two open states (approximately 5 and approximately 100 ms) and three closed states (< or = 5, approximately 100, and approximately 1,000 ms). Current noise analysis resulted in spectra containing two distinct Lorentzian noise components with corner frequencies of 1.3 Hz and approximately 50 Hz, respectively. Single-channel time constants were dependent on voltage. The fastest closed state increased its contribution from 48% at +100 mV to 87% at -100 mV, and the medium open state reduced its length to one half, resulting in gating dominated by fast events. Similarly, the fast Lorentzian increased its amplitude, and its corner frequency increased from 44 Hz at +100 mV to 91 Hz at -100 mV, while the slow Lorentzian was voltage independent. In multi-channel rec...Continue Reading

Citations

Sep 16, 2004·The Journal of Membrane Biology·Z R ZhangN A McCarty
Apr 20, 2010·Nature Cell Biology·Wei Kevin ZhangPingbo Huang
Nov 2, 2002·British Journal of Pharmacology·Xiandi GongPaul Linsdell
Mar 1, 1995·The Journal of General Physiology·B D SchultzR A Frizzell
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Apr 29, 1998·Annual Review of Physiology·J K Foskett
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