CFTR expression but not Cl- transport is involved in the stimulatory effect of bile acids on apical Cl-/HCO3- exchange activity in human pancreatic duct cells

Pancreas
Imre IgnáthZ Rakonczay

Abstract

Low doses of chenodeoxycholate (CDC) stimulate apical anion exchange and HCO3(-) secretion in guinea pig pancreatic duct cells (Gut. 2008;57:1102-1112). We examined the effects of CDC on intracellular pH (pHi), intracellular Ca(2+) concentration ([Ca(2+)]i), and apical Cl(-)/HCO3(-) exchange activity in human pancreatic duct cells and determined whether any effects were dependent on cystic fibrosis transmembrane conductance regulator (CFTR) expression and Cl(-) channel activity. Polarized CFPAC-1 cells (expressing F508del CFTR) were transduced with Sendai virus constructs containing complementary DNAs for either wild-type CFTR or beta-galactosidase. Microfluorimetry was used to record pHi and [Ca(2+)]i and apical Cl(-)/HCO3(-) exchange activity. Patch clamp experiments were performed on isolated guinea pig duct cells. Chenodeoxycholate induced a dose-dependent intracellular acidification and a marked increase in [Ca(2+)]i in CFPAC-1 cells. CFTR expression slightly reduced the rate of acidification but did not affect the [Ca(2+)]i changes. Luminal administration of 0.1 mmol/L of CDC significantly elevated apical Cl(-)/HCO3(-) exchange activity but only in cells that expressed CFTR. However, CDC did not activate CFTR Cl(-) conduc...Continue Reading

References

Jan 1, 1994·The American Journal of Physiology·M A GrayB E Argent
Mar 1, 1993·The American Journal of Physiology·M A GrayB E Argent
Oct 1, 1993·The American Journal of Physiology·D AlvaroJames L Boyer
Sep 6, 2000·Nature Biotechnology·Y YonemitsuEric W F W Alton
Nov 7, 2001·BMC Gastroenterology·M StelznerR Kuver
Oct 17, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Charles OkoloT D Nguyen
Mar 29, 2003·Physiological Reviews·Michael Trauner, James L Boyer
Apr 22, 2004·The Journal of Pharmacy and Pharmacology·J HardcastleC J Taylor
Feb 16, 2005·Annual Review of Physiology·M C StewardR Maynard Case
Jul 23, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Marcel J C BijveldsHugo R De Jonge
Nov 8, 2005·World Journal of Gastroenterology : WJG·P HegyiZsolt Boldogköi
Dec 13, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·L FischerStephen J Pandol
Mar 27, 2007·Gastroenterology·Stephen J PandolPeter A Banks
Apr 14, 2007·Cell Death and Differentiation·D N CriddleO V Gerasimenko
Jun 29, 2007·Gene Therapy·S FerrariEric W F W Alton
Jul 27, 2007·Journal of Cellular Physiology·Z RakonczayM A Gray
Jul 17, 2008·Gut·Min Goo Lee, S Muallem

Citations

Feb 19, 2010·American Journal of Physiology. Cell Physiology·Anurag Kumar SinghUrsula Seidler
Jul 25, 2015·Clinics and Research in Hepatology and Gastroenterology·Anita Balázs, P Hegyi
Apr 30, 2015·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·P Hegyi, Zoltan Rakonczay
Jun 9, 2015·Cell Communication and Signaling : CCS·Justyna M KowalI Novak
Oct 8, 2016·Cellular and Molecular Life Sciences : CMLS·Vinciane Saint-Criq, M A Gray
Dec 22, 2020·Journal of Hepatology·David C TrampertUlrich Beuers

Related Concepts

Carbonic Acid Ions
Calcium
Quenocol
Chloride Ion Level
Dose-Response Relationship, Drug
Cavia porcellus
Hydrogen-Ion Concentration
Resting Potentials
Structure of Accessory Pancreatic Duct
Malignant Neoplasm of Pancreas

Related Feeds

Carcinoma, Ductal

Ductal carcinoma is a malignant neoplasm involving the ductal systems of any of a number of organs, such as the mammary glands, pancreas, prostate or lacrimal gland. Discover the latest research on ductal carcinoma here.