CFTR involvement in nasal potential differences in mice and pigs studied using a thiazolidinone CFTR inhibitor

American Journal of Physiology. Lung Cellular and Molecular Physiology
Danieli B SalinasA S Verkman

Abstract

Nasal potential difference (PD) measurements have been used to demonstrate defective CFTR function in cystic fibrosis (CF) and to evaluate potential CF therapies. We used the selective thiazolidinone CFTR inhibitor CFTR(inh)-172 to define the involvement of CFTR in nasal PD changes in mice and pigs. In normal mice infused intranasally with a physiological saline solution containing amiloride, nasal PD was -4.7 +/- 0.7 mV, hyperpolarizing by 15 +/- 1 mV after a low-Cl- solution, and a further 3.9 +/- 0.5 mV after forskolin. CFTR(inh)-172 produced 1.1 +/- 0.9- and 4.3 +/- 0.7-mV depolarizations when added after low Cl- and forskolin, respectively. Systemically administered CFTR(inh)-172 reduced the forskolin-induced hyperpolarization from 4.7 +/- 0.4 to 0.9 +/- 0.1 mV but did not reduce the low Cl(-)-induced hyperpolarization. Nasal PD was -12 +/- 1 mV in CF mice after amiloride, changing by <0.5 mV after low Cl- or forskolin. In pigs, nasal PD was -14 +/- 3 mV after amiloride, hyperpolarizing by 13 +/- 2 mV after low Cl- and a further 9 +/- 1 mV after forskolin. CFTR(inh)-172 and glibenclamide did not affect nasal PD in pigs. Our results suggest that cAMP-dependent nasal PDs in mice primarily involve CFTR-mediated Cl- conductanc...Continue Reading

References

Jun 1, 1992·The American Journal of Physiology·M YamayaJ H Widdicombe
Sep 1, 1987·The Journal of Pediatrics·R A SauderG M Loughlin
Apr 1, 1984·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·M KnowlesR Boucher
May 1, 1983·The Journal of Clinical Investigation·M KnowlesR Boucher
Dec 17, 1981·The New England Journal of Medicine·M KnowlesR Boucher
May 1, 1994·The American Journal of Physiology·B R GrubbR C Boucher
Mar 1, 1995·Pflügers Archiv : European journal of physiology·A RabeE Frömter
Oct 1, 1995·The Journal of Clinical Investigation·B G ZeiherK R Thomas
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·T J KelleyM L Drumm
Apr 3, 1998·Human Gene Therapy·M RamjeesinghC E Bear
Oct 12, 1999·The American Journal of Physiology·S T BallardA Crews
Jun 19, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·H FischerM A Matthay
Nov 13, 2001·American Journal of Physiology. Renal Physiology·Y GuanM D Breyer
Jul 4, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Pamela L ZeitlinLois Brass-Ernst
Jun 28, 2003·American Journal of Respiratory and Critical Care Medicine·Helen L WallaceKevin W Southern
Aug 9, 2003·Chest·Michael P BoyleUNKNOWN Cystic Fibrosis Therapeutics Development Network Nasal PD Study Group
Oct 15, 2003·The Journal of General Physiology·Yuanlin SongA S Verkman
Mar 6, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jay R ThiagarajahA S Verkman

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Citations

Jul 22, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Estelle Cormet-BoyakaEric J Sorscher
Mar 1, 2006·Critical Care Medicine·Tsutomu SakumaMichael A Matthay
Aug 19, 2006·Respirology : Official Journal of the Asian Pacific Society of Respirology·Xiu GuTsutomu Sakuma
Jan 15, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Eric S SchiffhauerPamela L Zeitlin
May 20, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Christopher S RogersMichael J Welsh
Sep 23, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kelvin D MacDonaldPamela L Zeitlin
Sep 30, 2006·American Journal of Respiratory Cell and Molecular Biology·Xiaoming LiuJohn F Engelhardt
Jan 19, 2005·Respiratory Research·Xiaofei WangPaul M Quinton
Mar 19, 2013·PloS One·Emilie Lyne SaussereauIsabelle Sermet-Gaudelus
Dec 7, 2013·American Journal of Respiratory Cell and Molecular Biology·Ahmed LazrakSadis Matalon
Mar 12, 2005·Journal of Pharmaceutical Sciences·N D SonawaneA S Verkman
May 11, 2013·Respirology : Official Journal of the Asian Pacific Society of Respirology·Weizhong JinJinjun Jiang
May 27, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·A S Verkman
May 21, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yasutada AkibaJonathan D Kaunitz
Mar 28, 2008·The Biochemical Journal·Emanuela CaciLuis J V Galietta
Aug 4, 2019·Scientific Reports·Dawood KhanCatriona Kelly
Jun 5, 2021·Frontiers in Pharmacology·Nicole ReyneMartin Donnelley

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