CFTR regulates acute inflammatory responses in macrophages

QJM : Monthly Journal of the Association of Physicians
Z Gao, Xiao Su

Abstract

Mutation of cystic fibrosis transmembrane conductance regulator (CFTR) in the airway epithelial cells can lead to recurrent airway inflammation in cystic fibrosis (CF). Dysfunction of CFTR in neutrophils could contribute to LPS-induced acute lung inflammation. Deficiency of CFTR could also facilitate platelet aggregation and neutrophil-platelet interaction and promote inflammation. To study whether inhibition or mutation of CFTR in alveolar macrophages (AMs) or peritoneal macrophages (PMs) would promote their proinflammatory responses and whether dysfunction of CFTR would deteriorate acute E. coli-induced lung or peritoneal inflammation. Laboratory study. ELISA was used to determine production of proinflammatory cytokines in the CFTR inhibited or mutated macrophages under LPS challenge. Lung or peritoneum lavage was used to analyze proinflammatory parameters and cell differentiation. Excess lung water and lung vascular permeability were measured for evaluating severity of acute lung inflammation. Escherichia coli LPS simulation in AMs increased CFTR expression. Inhibition or mutation of CFTR in both AMs and PMs enhanced production of tumor necrosis factor alpha (TNF-α) and macrophage inflammatory protein-2 (MIP-2). Mutation of ...Continue Reading

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Citations

Sep 4, 2015·American Journal of Respiratory and Critical Care Medicine·Bob A LubambaCarla M P Ribeiro
Jun 15, 2016·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Don HayesDmitry Tumin
Sep 21, 2016·QJM : Monthly Journal of the Association of Physicians·Seamas C Donnelly
Jan 12, 2017·International Journal of Molecular Sciences·Carla M P Ribeiro, Bob A Lubamba
May 13, 2017·Médecine sciences : M/S·Bob A Lubamba
Apr 6, 2019·American Journal of Respiratory Cell and Molecular Biology·Sean V Murphy, Carla M P Ribeiro
Oct 15, 2021·The Journal of General Physiology·Daniel T InfieldNael A McCarty
Aug 12, 2019·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·M DeclercqP Witters

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