CG15031/PPYR1 is an intrinsically unstructured protein that interacts with protein phosphatase Y

Archives of Biochemistry and Biophysics
Endre KókaiViktor Dombrádi

Abstract

Protein phosphatase Y (PPY) is a Drosophila testis-specific enzyme of unknown function. In a yeast two-hybrid screen we identified CG15031/PPYR1 as a PPY interacting protein. The specificity of the protein-protein interaction was proven by directed two-hybrid tests. The complex formation between PPY and PPYR1 was confirmed under in vitro and in vivo conditions by plasmon resonance spectroscopy, co-immunoprecipitation, and pull down experiments. Recombinant PPYR1 expressed in Escherichia coli is a heatstable, protease sensitive, intrinsically unstructured RNA-binding protein that migrates anomalously in SDS-polyacrylamide gel electrophoresis. It can be phosphorylated by cAMP-dependent protein kinase in vitro. PPYR1 moderately inhibits PPY activity, the inhibitory potential of the protein is slightly increased by phosphorylation. We suggest that PPYR1 may function as a scaffolding protein that targets PPY to RNA and other protein partners in Drosophila melanogaster.

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Dec 13, 2007·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Jul 16, 2008·Molecular and Cellular Biology·Yifei Wang, Mary Ann Price
Mar 25, 2011·Metallomics : Integrated Biometal Science·Md Khurshid Alam KhanFaizan Ahmad
Oct 10, 2006·Journal of Molecular Biology·Rajesh KumarAbani K Bhuyan

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