cGAS/STING/TBK1/IRF3 Signaling Pathway Activates BMDCs Maturation Following Mycobacterium bovis Infection

International Journal of Molecular Sciences
Qiang LiLihua Xu

Abstract

Cyclic GMP-AMP synthase (cGAS) is an important cytosolic DNA sensor that plays a crucial role in triggering STING-dependent signal and inducing type I interferons (IFNs). cGAS is important for intracellular bacterial recognition and innate immune responses. However, the regulating effect of the cGAS pathway for bone marrow-derived dendritic cells (BMDCs) during Mycobacterium bovis (M. bovis) infection is still unknown. We hypothesized that the maturation and activation of BMDCs were modulated by the cGAS/STING/TBK1/IRF3 signaling pathway. In this study, we found that M. bovis promoted phenotypic maturation and functional activation of BMDCs via the cGAS signaling pathway, with the type I IFN and its receptor (IFNAR) contributing. Additionally, we showed that the type I IFN pathway promoted CD4⁺ T cells' proliferation with BMDC during M. bovis infection. Meanwhile, the related cytokines increased the expression involved in this signaling pathway. These data highlight the mechanism of the cGAS and type I IFN pathway in regulating the maturation and activation of BMDCs, emphasizing the important role of this signaling pathway and BMDCs against M. bovis. This study provides new insight into the interaction between cGAS and dendriti...Continue Reading

References

Jan 8, 2003·The Journal of Experimental Medicine·Ludovic TailleuxOlivier Neyrolles
Feb 8, 2005·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·E ErsoyY Zorlu
Feb 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sarah A StanleyJeffery S Cox
Nov 1, 2007·European Journal of Immunology·Ralph M Steinman
Mar 15, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Eui-Hong ByunSung Jae Shin
Jul 28, 2012·Current Medicinal Chemistry·M HansenM H Andersen
Jun 1, 2013·Nature·Filiz CivrilKarl-Peter Hopfner
Jun 6, 2013·Emerging Infectious Diseases·Borna MüllerJakob Zinsstag
Jul 6, 2013·European Journal of Immunology·Karin Pelka, Eicke Latz
Nov 8, 2013·Journal of Virology·Eric LamErik Falck-Pedersen
Nov 2, 2014·Cold Spring Harbor Perspectives in Medicine·Philippe GlaziouMario Raviglione
Dec 3, 2014·Seminars in Immunology·Marilena Paola EtnaEliana Marina Coccia
Jan 23, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Feng MaGenhong Cheng
Jun 7, 2015·Cell Host & Microbe·Angela C CollinsMichael U Shiloh
Jun 15, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Colleen ScottPatrick K Moonan
Jul 14, 2016·Oncotarget·Chunfa LiuXiangmei Zhou

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Methods Mentioned

BETA
transfection
targeted knockdown
flow cytometry
immunoprecipitation assay
enzyme-linked immunosorbent assay

Software Mentioned

SPSS
FlowJo
GraphPad Prism

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