PMID: 8962144Dec 10, 1996Paper

CGM1a antigen of neutrophils, a receptor of gonococcal opacity proteins

Proceedings of the National Academy of Sciences of the United States of America
T Chen, E C Gotschlich

Abstract

Neisseria gonorrhoeae (GC) or Escherichia coli expressing phase-variable opacity (Opa) protein (Opa+ GC or Opa+ E. coli) adhere to human neutrophils and stimulate phagocytosis, whereas their counterparts not expressing Opa protein (Opa- GC or Opa- E. coli) do not. Opa+ GC or E. coli do not adhere to human lymphocytes and promyelocytic cell lines such as HL-60 cells. The adherence of Opa+ GC to the neutrophils can be enhanced dramatically if the neutrophils are preactivated. These data suggest that the components binding the Opa+ bacteria might exist in the granules. CGM1a antigen, a transmembrane protein of the carcinoembryonic antigen family, is exclusively expressed in the granulocytic lineage. The predicted molecular weight of CGM1a is approximately 30 kDa. We observed specific binding of OpaI+ E. coli to a 30-kDa band of polymorphonuclear leukocytes lysates. To prove the hypothesis that the 30-kDa CGM1a antigen from neutrophils was the receptor of Opa+ bacteria, we showed that a HeLa cell line expressing human CGM1a antigen (HeLa-CGM1a) bound Opa+ E. coli and subsequently engulfed the bacteria. Monoclonal antibodies (COL-1) against CGM1 blocked the interaction between Opa+ E. coli and HeLa-CGM1a. These results demonstrate t...Continue Reading

References

Jan 1, 1979·Methods in Enzymology·R R Fall
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Aug 1, 1977·The Journal of Infectious Diseases·B A Evans
Nov 1, 1975·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·M BordesF Martin
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D Simon, R F Rest
Jul 1, 1992·Molecular Microbiology·R J BellandS H Fischer
Jan 1, 1991·Infection·J E Fowler
Jan 1, 1990·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·T Barnett, W Zimmermann
May 1, 1987·The Journal of Experimental Medicine·J SwansonJ M Koomey
Dec 1, 1988·The Journal of Experimental Medicine·J SwansonJ Boslego
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R J PaxtonJ E Shively
Sep 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·S von KleistP Burtin
Jun 1, 1974·The Journal of Infectious Diseases·M E WardJ N Robertson
Apr 1, 1970·Biulleten' eksperimental'noĭ biologii i meditsiny
Mar 1, 1981·Infection and Immunity·M Virji, J S Everson
Aug 1, 1995·The Journal of Experimental Medicine·T ChenJ Swanson
Aug 26, 1994·Cell·J V Ravetch
Mar 1, 1994·The Journal of Experimental Medicine·A E JerseJ G Cannon
Apr 1, 1994·The Journal of Clinical Investigation·F A PlummerR C Brunham
Jan 1, 1996·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·F PrallC Wagener
Jun 15, 1996·Journal of Cellular Biochemistry·J R Couchman, A Woods
Mar 1, 1995·Trends in Cell Biology·S Greenberg

❮ Previous
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Citations

Jun 22, 1999·Immunologic Research·K M Coggeshall
Aug 26, 1998·Folia Microbiologica·T F Meyer
Oct 2, 2003·Current Opinion in Cell Biology·Erin C Boyle, B Brett Finlay
Mar 5, 2003·Current Opinion in Microbiology·Christof R Hauck, Thomas F Meyer
Feb 27, 1999·Current Opinion in Microbiology·M NaumannT F Meyer
Mar 17, 2009·Nature Reviews. Microbiology·Mumtaz Virji
Feb 1, 2012·Nature Reviews. Microbiology·Alison K Criss, H Steven Seifert
Sep 6, 2012·The Journal of Biological Chemistry·Kathrin KoppChristof R Hauck
Jan 7, 2004·The Journal of Experimental Medicine·Phillip Henneke, Douglas T Golenbock
Apr 8, 1999·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·T F Meyer
Apr 7, 2006·Applied and Environmental Microbiology·Silvia BulgheresiJörg A Ott
Jul 20, 2004·Clinical Microbiology Reviews·Marjan W van der Woude, Andreas J Bäumler
Oct 19, 2004·Clinical Microbiology Reviews·Jennifer L Edwards, Michael A Apicella
Aug 19, 2000·Infection and Immunity·J M Spence, V L Clark
Sep 13, 2001·Infection and Immunity·A RytkönenA B Jonsson

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