cGMP accumulation and gene expression of soluble guanylate cyclase in human vascular tissue

Journal of Cellular Physiology
A PapapetropoulosJ D Catravas

Abstract

Gene expression of soluble guanylate cyclase (sGC) and cGMP accumulation in response to sodium nitroprusside (SNP) were studied in cultured human vascular cells and freshly harvested vascular tissue. As revealed by reverse transcriptase-polymerase chain reaction, cultured smooth muscle and endothelial cells, as well as freshly isolated human vascular tissue, express mRNA for the alpha 3 and beta 3 subunits but not for the alpha 2 and beta 3 subunits is evident even in the absence of increased cGMP accumulation in response to SNP. cGMP accumulation in human cells cultured from different vascular beds typically increased two- to five-fold (maximum of 11.4-fold) over baseline following stimulation with 100 microM SNP. Bovine, murine, canine, and avian vascular smooth muscle cells accumulated similar or lower amounts of cGMP than human cells, whereas porcine, rat, and rabbit smooth muscle cells accumulated greater amounts of cGMP. In freshly harvested human vessels, cGMP accumulation in response to SNP was found to increase fifteen-fold over baseline. In contrast to the SNP-induced cGMP accumulation, cGMP levels in response to particulate guanylate cyclase activator atriopeptin II were equal to or greater in cultured human cells th...Continue Reading

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Citations

Jul 27, 2000·Journal of Cellular Physiology·J A CarvajalC P Weiner
Apr 19, 2011·Angiogenesis·Anna Maria CuratolaKaren D Hendricks-Munoz
May 26, 1998·European Journal of Pharmacology·A PapapetropoulosJ D Catravas
Feb 12, 1998·Trends in Pharmacological Sciences·A J Hobbs
Aug 28, 2003·Clinical and Experimental Pharmacology & Physiology·Francis I Achike, Chiu-Yin Kwan
May 2, 2009·Journal of Applied Physiology·William J PearceThomas M Lincoln
Jul 9, 1998·American Journal of Respiratory and Critical Care Medicine·K A HansonW R Clarke
Jul 12, 2013·American Journal of Respiratory and Critical Care Medicine·Constantinos GlynosGuy G Brusselle
Mar 4, 2000·The Biochemical Journal·D RitterP Y Kwok
Oct 20, 2007·Life Sciences·Anastasia PyriochouAndreas Papapetropoulos
Jul 2, 2004·International Journal of Impotence Research·I F Ghalayini
May 30, 2003·Pharmacological Reviews·Noboru Toda, Tomio Okamura
Aug 31, 2006·The Journal of Pharmacology and Experimental Therapeutics·Anastasia PyriochouAndreas Papapetropoulos

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