ChAdOx1 nCoV-19 vaccine prevents SARS-CoV-2 pneumonia in rhesus macaques.

Nature
Neeltje van DoremalenVincent J Munster

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in December 20191,2 and is responsible for the coronavirus disease 2019 (COVID-19) pandemic3. Vaccines are an essential countermeasure and are urgently needed to control the pandemic4. Here we show that the adenovirus-vector-based vaccine ChAdOx1 nCoV-19, which encodes the spike protein of SARS-CoV-2, is immunogenic in mice and elicites a robust humoral and cell-mediated response. This response was predominantly mediated by type-1 T helper cells, as demonstrated by the profiling of the IgG subclass and the expression of cytokines. Vaccination with ChAdOx1 nCoV-19 (using either a prime-only or a prime-boost regimen) induced a balanced humoral and cellular immune response of type-1 and type-2 T helper cells in rhesus macaques. We observed a significantly reduced viral load in the bronchoalveolar lavage fluid and lower respiratory tract tissue of vaccinated rhesus macaques that were challenged with SARS-CoV-2 compared with control animals, and no pneumonia was observed in vaccinated SARS-CoV-2-infected animals. However, there was no difference in nasal shedding between vaccinated and control SARS-CoV-2-infected macaques. Notably, we found no evidence of immune-en...Continue Reading

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Jun 25, 2020·Science Advances·Neeltje van DoremalenVincent J Munster

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Citations

Sep 25, 2020·Current Opinion in HIV and AIDS·Yihan WangDongming Zhou
Sep 24, 2020·Nature·Florian Krammer
Aug 28, 2020·F1000Research·Wern Hann NgSuresh Mahalingam
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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
Assay
ELISA

Software Mentioned

Graphpad Prism
CTL ImmunoSpot Analyzer
TreeStar
FlowJo
ImmunoSpot Software
GeneArt Gene

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