Chaetocin enhances dendritic cell function via the induction of heat shock protein and cancer testis antigens in myeloma cells

Oncotarget
Manh-Cuong VoJe-Jung Lee

Abstract

Dendritic cells (DC)-based vaccines are considered useful in cancer immuno-therapy, and the interactions of DC and dying tumor cells are important and promising for cancer immunotherapy. We investigated whether chaetocin could be used to induce death of myeloma cells, for loading onto DCs can affect DCs function. In this study, we show that the dying myeloma cells treated with chaetocin resulted in the induction of heat shock protein (HSP) 90, which was inhibited by antioxidant N-acetyl cysteine, and showed an increase in the expression of MAGE-A3 and MAGE-C1/CT7. DCs loaded with chaetocin-treated dying myeloma cells produced low levels of IL-10 and enhanced the cross presentation of DCs. Additionally, these DCs most potently inhibited regulatory T cells, induced Th1 polarization and activated myeloma-specific cytotoxic T lymphocytes compared with DCs loaded with UVB-irradiated dying myeloma cells. These results suggest that the pretreatment of myeloma cells with chaetocin can enhance DC function through the up-regulation of HSP90 and cancer testis antigens in dying myeloma cells and can potently induce the Th1 polarization of DCs and myeloma-specific cytotoxic T lymphocytes.

References

Aug 1, 1981·Canadian Journal of Microbiology·S SekitaM Umeda
Apr 1, 1998·Nature·J Banchereau, R M Steinman
Apr 12, 2001·British Journal of Haematology·D C Reid
Oct 29, 2002·Annals of Oncology : Official Journal of the European Society for Medical Oncology·J Drach, H Kaufmann
Apr 26, 2003·Blood·Toshiaki HayashiKenneth C Anderson
Dec 3, 2003·Cancer Immunology, Immunotherapy : CII·Lee D CranmerEvan M Hersh
Jan 23, 2004·Cancer Investigation·Derry Ridgway
Apr 17, 2004·British Journal of Haematology·Noopur RajeKenneth C Anderson
May 4, 2004·Nature Medicine·Carl G FigdorCornelis J M Melief
Jul 13, 2004·Advances in Immunology·Laurence ZitvogelGuido Kroemer
May 3, 2005·Nature Reviews. Cancer·Richard A Lake, Bruce W S Robinson
Jul 27, 2005·British Journal of Haematology·S J Harrison, G Cook
Dec 21, 2005·The Journal of Experimental Medicine·Noelia CasaresGuido Kroemer
Jan 13, 2006·Nature Chemical Biology·Dorothea GreinerAxel Imhof
May 19, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Robert C KaneRichard Pazdur
Jun 9, 2006·The New England Journal of Medicine·Richard A Lake, Robbert G van der Most
Dec 22, 2006·Expert Review of Anticancer Therapy·Simon J HarrisonH Miles Prince
Sep 6, 2007·Epigenetics : Official Journal of the DNA Methylation Society·Adam R Karpf
Mar 3, 2009·Advanced Drug Delivery Reviews·Bernadett Kalmar, Linda Greensmith
Apr 7, 2012·Clinical & Developmental Immunology·Thanh-Nhan Nguyen-PhamJe-Jung Lee
Mar 13, 2014·Methods in Molecular Biology·Hyun-Ju LeeJe-Jung Lee
Apr 2, 2014·Leukemia & Lymphoma·Shaji K Kumar, Morie A Gertz
Apr 29, 2015·Chonnam Medical Journal·My-Dung HoangJe-Jung Lee

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Citations

Mar 1, 2018·Annual Review of Immunology·J Magarian Blander
Feb 16, 2019·Epigenetics : Official Journal of the DNA Methylation Society·Ashok SharmaAdam R Karpf
Feb 14, 2019·The Korean Journal of Internal Medicine·Manh-Cuong VoJe-Jung Lee
Aug 20, 2020·Technology in Cancer Research & Treatment·Zhaoyu LiuYongheng Yang
Aug 21, 2018·Frontiers in Immunology·Manh-Cuong VoJe-Jung Lee
Sep 1, 2021·European Journal of Pharmacology·Hangyu JiangLin Li

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Methods Mentioned

BETA
flow cytometry
electrophoresis
enzyme-linked immunosorbent assay
FACS

Software Mentioned

SPSS
WinMDI

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