Chaga mushroom (Inonotus obliquus) induces G0/G1 arrest and apoptosis in human hepatoma HepG2 cells.

World Journal of Gastroenterology : WJG
Myung-Ja YounRaekil Park

Abstract

To investigate the anti-proliferative and apoptotic effects of Chaga mushroom (Inonotus obliquus) water extract on human hepatoma cell lines, HepG2 and Hep3B cells. The cytotoxicity of Chaga extract was screened by 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay. Morphological observation, flow cytometry analysis, Western blot were employed to elucidate the cytotoxic mechanism of Chaga extract. HepG2 cells were more sensitive to Chaga extract than Hep3B cells, as demonstrated by markedly reduced cell viability. Chaga extract inhibited the cell growth in a dose-dependent manner, which was accompanied with G0/G1-phase arrest and apoptotic cell death. In addition, G0/G1 arrest in the cell cycle was closely associated with down-regulation of p53, pRb, p27, cyclins D1, D2, E, cyclin-dependent kinase (Cdk) 2, Cdk4, and Cdk6 expression. Chaga mushroom may provide a new therapeutic option, as a potential anticancer agent, in the treatment of hepatoma.

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Citations

Jul 28, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Jin-Yi WuChing-Hsein Chen
May 4, 2012·World Journal of Gastroenterology : WJG·Chun-Ping JiangJun-Hua Wu
Jul 24, 2015·International Journal of Molecular Medicine·Zengmei LanJun Zhou
Feb 11, 2016·Complementary Therapies in Medicine·Hyun-Mok JuKi Han Kwon
Jul 10, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Pei-Jane HuangHsin-Ling Yang
May 18, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Fu-Qiang SongGe Song
Aug 27, 2016·Biomedit︠s︡inskai︠a︡ khimii︠a︡·S A NikitinaM A Sysoeva
May 18, 2020·International Journal of Molecular Sciences·Anna SadowskaHalina Car
Nov 18, 2020·Journal of Fungi·Lan Lin, Jianping Xu
May 16, 2021·International Journal of Biological Macromolecules·Shoib Mohmad WaniFirdous Ahmad Khanday
Jul 2, 2021·Journal of Traditional and Complementary Medicine·Konrad A SzychowskiJan Gmiński

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