PMID: 11309138Apr 20, 2001Paper

Chain-length specificities of maize starch synthase I enzyme: studies of glucan affinity and catalytic properties

The Plant Journal : for Cell and Molecular Biology
P D Commuri, P L Keeling

Abstract

It is widely known that some of the starch synthases and starch-branching enzymes are trapped inside the starch granule matrix during the course of starch deposition in amyloplasts. The objective of this study was to use maize SSI to further our understanding of the protein domains involved in starch granule entrapment and identify the chain-length specificities of the enzyme. Using affinity gel electrophoresis, we measured the dissociation constants of maize SSI and its truncated forms using various glucans. The enzyme has a high degree of specificity in terms of its substrate-enzyme dissociation constant, but has a greatly elevated affinity for increasing chain lengths of alpha-1, 4 glucans. Deletion of the N-terminal arm of SSI did not affect the Kd value. Further small deletions of either N- or C-terminal domains resulted in a complete loss of any measurable affinity for its substrate, suggesting that the starch-affinity domain of SSI is not discrete from the catalytic domain. Greater affinity was displayed for the amylopectin fraction of starch as compared to amylose, whereas glycogen revealed the lowest affinity. However, when the outer chain lengths (OCL) of glycogen were extended using the phosphorylase enzyme, we found...Continue Reading

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Citations

May 20, 2003·Current Opinion in Plant Biology·Martha G JamesAlan M Myers
Jan 31, 2006·Plant Physiology·Naoko FujitaYasunori Nakamura
Aug 13, 2002·The Plant Cell·Anne EdwardsCathie Martin
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Apr 20, 2010·Plant Physiology and Biochemistry : PPB·Jong-Seong JeonYasunori Nakamura
Jul 28, 2005·The Plant Journal : for Cell and Molecular Biology·David DelvalléChristophe D'Hulst
Aug 8, 2007·The FEBS Journal·Takeshi SenouraHirokazu Matsui
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Sep 27, 2020·International Journal of Molecular Sciences·Ian J Tetlow, Eric Bertoft
Nov 25, 2020·The Plant Journal : for Cell and Molecular Biology·Sahar MehrpouyanMichael J Emes

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