Chain ordering of Stratum corneum lipids investigated by EPR slow-tumbling simulation

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Kouichi NakagawaH I Maibach

Abstract

We investigated the chain ordering of the lipid bilayer of Stratum corneum (SC) using an electron paramagnetic resonance (EPR) spin probe method in conjunction with slow-tumbling simulation. The ordering of SC lipids was evaluated by analysis of the signals of 5-doxylstearic acid (5-DSA) spin probe incorporated into the lamellar lipids. The result obtained with the conventional method of calculating the order parameter using hyperfine values was 0.80. The value of the order parameter obtained by spectral simulation was 0.73. It was found that the conventional method of calculating the chain ordering using hyperfine values could not differentiate subtle EPR spectral changes. However, EPR slow-tumbling simulation can differentiate such subtle spectral changes. Thus, the present EPR investigation suggests that simulation provides more detail about the structure of the lipid bilayer than the conventional method.

References

Jun 29, 2001·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Junichi MizushimaHoward I. Maibach

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Citations

Feb 7, 2012·Journal of Dermatological Science·Kouichi NakagawaDaisuke Sawamura
Nov 18, 2006·The Journal of Investigative Dermatology·Eiichiro YagiKouichi Nakagawa
Jan 14, 2011·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Kouichi Nakagawa
Oct 30, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kouichi NakagawaDaisuke Sawamura
Dec 12, 2017·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kouichi NakagawaHideyuki Hara

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