Chalcone-Induced Apoptosis through Caspase-Dependent Intrinsic Pathways in Human Hepatocellular Carcinoma Cells

International Journal of Molecular Sciences
Rodrigo Ramirez-TagleCesar Echeverria

Abstract

Hepatocellular carcinoma (HCC) is one of the most commonly diagnosed cancers worldwide. Chemoprevention of HCC can be achieved through the use of natural or synthetic compounds that reverse, suppress or prevent the development of cancer progression. In this study, we investigated the antiproliferative effects and the mechanism of action of two compounds, 2,3,4'-trimethoxy-2'-hydroxy-chalcone (CH1) and 3'-bromo-3,4-dimethoxy-chalcone (CH2), over human hepatoma cells (HepG2 and Huh-7) and cultured mouse hepatocytes (HepM). Cytotoxic effects were observed over the HepG2 and Huh-7, and no effects were observed over the HepM. For HepG2 cells, treated separately with each chalcone, typical apoptotic laddering and nuclear condensation were observed. Additionally, the caspases and Bcl-2 family proteins activation by using Western blotting and immunocytochemistry were studied. Caspase-8 was not activated, but caspase-3 and -9 were both activated by chalcones in HepG2 cells. Chalcones also induced reactive oxygen species (ROS) accumulation after 4, 8 and 24 h of treatment in HepG2 cells. These results suggest that apoptosis in HepG2 was induced through: (i) a caspase-dependent intrinsic pathway; and (ii) by alterations in the cellular le...Continue Reading

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Citations

Feb 27, 2020·Future Medicinal Chemistry·Mohamed I ChouiterArtur Ms Silva
May 24, 2019·Journal of Enzyme Inhibition and Medicinal Chemistry·Mariana Bastos Dos SantosLuis Octavio Regasini
Nov 21, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rimsha IrfanRahman Shah Zaib Saleem
Jan 9, 2021·BioMed Research International·Wasitta Rachakhom, Ratana Banjerdpongchai
Aug 28, 2021·Biomolecules·Hiba A JasimSatyajit D Sarker
Jun 2, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Naiwei DongBaofeng Yang

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Methods Mentioned

BETA
surgical resection
flow cytometry
electrophoresis

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