Challenge in direct Spoligotyping of Mycobacterium tuberculosis: a problematic issue in the region with high prevalence of polyclonal infections

BMC Research Notes
Mansour Kargarpour KamakoliFarzam Vaziri

Abstract

Based on our recent studies the prevalence of polyclonal infection in tuberculosis clinical specimens is more than 50% in Tehran, Iran. With this background, Spoligotyping was performed on clinical specimens and their respective cultures, and we examined whether mixed infections interfere with the results or not. Based on the Spoligotyping pattern, among the fourteen patients, 57.1% had different genotypes in clinical samples and their respective cultures. These discrepant patterns were suggestive of polyclonal infections in clinical samples with possible overlapping Spoligotype patterns. We propose that in societies with high mixed infections (e.g. Iran), direct Spoligotyping on clinical samples can be controversial.

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Citations

Jul 18, 2019·Infectious Diseases·Mansour Kargarpour KamakoliFarzam Vaziri
Jan 16, 2020·Molecular Biology Reports·Mansour Kargarpour KamakoliFarzam Vaziri
Jan 8, 2021·Frontiers in Genetics·Alexander Stephen ByrneKapil Tahlan
Nov 16, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Mebrat EjoBouke C de Jong
Aug 10, 2021·Frontiers in Public Health·Prince AsareDorothy Yeboah-Manu

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Methods Mentioned

BETA
genotyping
PCR

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