Challenges to accurate susceptibility testing and interpretation of quinolone resistance in Enterobacteriaceae: results of a Spanish multicentre study

The Journal of Antimicrobial Chemotherapy
José-Manuel Rodriguez-MartinezAlvaro Pascual

Abstract

The objective of this study was to evaluate the proficiency of Spanish laboratories with respect to accurate susceptibility testing and the detection and interpretation of quinolone resistance phenotypes in Enterobacteriaceae. Thirteen strains of Enterobacteriaceae were sent to 62 participating centres throughout Spain; strains harboured GyrA/ParC modifications, reduced permeability and/or plasmid-mediated quinolone resistance genes. The centres were requested to evaluate nalidixic acid and five quinolones, provide raw/interpreted clinical categories and to detect/infer resistance mechanisms. Consensus results from reference centres were used to assign minor, major and very major errors (mEs, MEs and VMEs, respectively). Susceptibility testing in the participating centres was frequently performed using the MicroScan WalkAway, Vitek 2 and Wider systems (48%, 30% and 8%, respectively). CLSI/EUCAST breakpoints were used in 71%/29% of the determinations. The percentage of VMEs for all quinolones was well below 2%. Only ofloxacin and moxifloxacin showed higher values for raw VMEs (6.6%), which decreased to 0% and 2.9%, respectively, in the interpreted VMEs. These errors were particularly associated with the CC-03 strain [qnrS2 + aac...Continue Reading

References

Jul 14, 1998·Antimicrobial Agents and Chemotherapy·L Martínez-MartínezA Pascual
Apr 11, 2001·Emerging Infectious Diseases·D C Hooper
Oct 2, 2003·The Annals of Pharmacotherapy·Jerome J SchentagAlan Forrest
Apr 12, 2005·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J M Rodríguez-MartinezA Pascual
Jun 9, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·George A Jacoby
Jun 19, 2008·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J M Rodríguez-MartínezL Martínez-Martínez
Sep 9, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·David L Paterson
Sep 15, 2009·Current Topics in Medicinal Chemistry·Karl DrlicaXilin Zhao
Oct 14, 2009·Clinical Microbiology Reviews·Jacob StrahilevitzAri Robicsek
Oct 5, 2010·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·José Manuel Rodríguez-MartínezAlvaro Pascual
Nov 28, 2012·Enfermedades infecciosas y microbiología clínica·Lorena López-CereroAlvaro Pascual
Oct 5, 2013·Diagnostic Microbiology and Infectious Disease·Agnese LupoAndrea Endimiani
Oct 8, 2013·Critical Care Clinics·Henry Fraimow, Raquel Nahra
Nov 26, 2013·Enfermedades infecciosas y microbiología clínica·Luis Martínez-MartínezRafael Cantón
Aug 21, 2014·The Journal of Antimicrobial Chemotherapy·Jesús MachucaJosé-Manuel Rodríguez-Martínez

❮ Previous
Next ❯

Citations

Jan 26, 2016·Journal of Microbiological Methods·S DinarelliG Longo
Sep 15, 2017·Microbes and Environments·Ana UmpiérrezPablo Zunino

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.