Chamber centrifugation: a harvesting technique for estimation of the growth of human haemopoietic cells in diffusion chambers

British Journal of Haematology
N Jacobsen

Abstract

A harvesting technique which gives information about the spatial relationships between cells grown in diffusion chambers in mice has been designed. The principle is that cells are centrifuged directly from the chamber clot onto a slide during pronase treatment. The technique has been applied to normal human bone marrow cells grown in diffusion chambers. The slides show that the cells are situated in clusters. A limiting dilution assay of progenitor cells based on the presence of granulopoietic clusters in the chambers demonstrates the use of the method for quantitation of progenitor cells.

References

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Aug 1, 1971·Journal of Cellular Physiology·H BreivikA Boyum
Aug 1, 1970·Journal of Cellular Physiology·B L Pike, W A Robinson
Dec 1, 1963·British Journal of Cancer·E H PORTER, R J BERRY

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Citations

May 1, 1976·Journal of Dentistry·T Low, J A von Fraunhofer
Nov 1, 1989·International Journal of Cell Cloning·E NiskanenM Symann
Jun 1, 1975·Archives of Biochemistry and Biophysics·P M DiGirolamo, F M Huennekens
Jan 1, 1985·European Journal of Cancer & Clinical Oncology·R WillemzeH Journée
Jan 1, 1981·American Journal of Hematology·K A FoonP I Terasaki
Jul 1, 1979·Scandinavian Journal of Haematology·N Jacobsen, H E Broxmeyer

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