PMID: 18724398Aug 30, 2008Paper

Chances and pitfalls of chemical cross-linking with amine-reactive N-hydroxysuccinimide esters

Analytical and Bioanalytical Chemistry
Stefan Kalkhof, Andrea Sinz

Abstract

In this report we summarize our experiences with the reaction products of N-hydroxysuccinimide (NHS) esters, which are widely used for chemical cross-linking of lysine residues in proteins. We describe the products, which should be scrutinized during data analysis using customized software when NHS esters are employed for chemical cross-linking. Reaction products of NHS esters were observed not only with lysines, but also with serines, tyrosines, and threonines. This report is intended to be a practical guide for those working in the field of chemical cross-linking and mass spectrometry.

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Citations

Sep 28, 2013·Analytical and Bioanalytical Chemistry·Sabine HerbstAndrea Sinz
Dec 2, 2011·Journal of the American Society for Mass Spectrometry·Billy Clifford-NunnPhilip C Andrews
Feb 21, 2013·Journal of the American Society for Mass Spectrometry·Eric D MerkleyJoshua N Adkins
Aug 17, 2010·Journal of the American Society for Mass Spectrometry·Stefanie MädlerRenato Zenobi
Sep 25, 2010·Journal of the American Society for Mass Spectrometry·Luiz F A SantosFabio C Gozzo
Oct 18, 2008·Bioconjugate Chemistry·Giselle M KnudsenV Jo Davisson
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Nir KalismanMichael Levitt
Jul 14, 2010·Molecular & Cellular Proteomics : MCP·Evgeniy V PetrotchenkoChristoph H Borchers
Dec 15, 2011·Molecular & Cellular Proteomics : MCP·Ignasi FornéFelix Mueller-Planitz
Apr 3, 2010·Molecular & Cellular Proteomics : MCP·Alexander LeitnerRuedi Aebersold
Apr 28, 2010·Nucleic Acids Research·Eva Kühn-HölskenHenning Urlaub
Jul 18, 2014·Journal of the American Society for Mass Spectrometry·Jens PettelkauAndrea Sinz
May 7, 2015·Mass Spectrometry Reviews·Fan ChenRenato Zenobi
Sep 24, 2015·Journal of Photochemistry and Photobiology. B, Biology·Terry M BrickerLaurie K Frankel
Jan 10, 2009·Journal of Mass Spectrometry : JMS·Stefanie MädlerRenato Zenobi
Aug 24, 2010·Mass Spectrometry Reviews·Evgeniy V Petrotchenko, Christoph H Borchers
Dec 15, 2012·Rapid Communications in Mass Spectrometry : RCM·Antonio N CalabreseTara L Pukala
Jul 17, 2010·Journal of Mass Spectrometry : JMS·Alexandre F Gomes, Fabio C Gozzo
Jul 17, 2013·Rapid Communications in Mass Spectrometry : RCM·Alena Dreiling, Simone König
Nov 11, 2009·Proteomics·David ParamelleGilles Subra
Feb 6, 2014·Rapid Communications in Mass Spectrometry : RCM·Ekaterina DariiJean-Claude Tabet
Oct 13, 2015·Cell Reports·Shelly RozenMichal Sharon
May 15, 2015·Protein Science : a Publication of the Protein Society·Andrea SinzMichal Sharon
Nov 26, 2009·Analytical Biochemistry·Stefanie MädlerRenato Zenobi
Jun 17, 2015·Methods : a Companion to Methods in Enzymology·Andrew N Holding
Oct 29, 2014·Proceedings of the National Academy of Sciences of the United States of America·Manjula P MummadisettiTerry M Bricker
Nov 19, 2014·Journal of the American Society for Mass Spectrometry·Fan ChenRenato Zenobi

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