Change in intracellular pH in Tetrahymena pyriformis GL in relation to the toxicity level of the lonophorous antibiotic nigericin

European Journal of Protistology
M BamdadM Gendraud


Nigericin, a carboxylic poly ether antibiotic, is toxic towards Tetrahymena pyriformis GL [1, 7, 12, 13]. Nigericin transports K(+) across biological membranes along the gradient. This transport is generally equilibrated by an influx of H(+) ions. The toxicity of nigericin (at low 10 mg · l(-1) and high 30 mg · l(-1) levels) was studied by measuring the intracellular pH of Tetrahymena pyriformis GL by means of two labelled molecules: (14)C-DMO and (3)H-inulin. This measurement of pHi requires prior knowledge of the number of cells and their volume. The results reveal a correlation of the toxicity of nigericin, acidification of the intracellular environment and increase in cell volume leading to the return of pHi to control values.


Dec 1, 1995·Applied Microbiology and Biotechnology·M BamdadC A Grolière
Mar 10, 1999·Chemosphere·M P SauvantE Piccinni

Related Concepts

Tetrahymena pyriformis
Fiber-forming protein, Tetrahymena pyriformis
Toxic Effect

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