Change in substrate preference of Streptomyces aminopeptidase through modification of the environment around the substrate binding site

Applied and Environmental Microbiology
Jiro ArimaTadashi Hatanaka

Abstract

We attempted to alter the substrate preference of aminopeptidase from Streptomyces septatus TH-2 (SSAP). Because Asp198 and Phe221 of SSAP are located in the substrate binding site, we screened 2,000 mutant enzymes with D198X/F221X mutations. By carrying out this examination, we obtained two enzymes; one specifically hydrolyzed an arginyl derivative, and the other specifically hydrolyzed a cystinyl derivative (65- and 12.5-fold higher k(cat) values for hydrolysis of p-nitroanilide derivatives than those of the wild type, respectively).

References

Jan 1, 1976·Methods in Enzymology·J M Prescott, S H Wilkes
Dec 1, 1988·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·G J SanderinkG Siest
Feb 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A Taylor
Jan 29, 1996·FEBS Letters·I VenekeiW J Rutter
Feb 7, 1997·Journal of Molecular Biology·H M GreenblattGil Shoham
May 1, 1999·The Journal of Biological Chemistry·K W Walker, R A Bradshaw
Dec 14, 1999·Protein Science : a Publication of the Protein Society·D Mahadevan, J W Saldanha
Apr 20, 2000·Acta Crystallographica. Section D, Biological Crystallography·Rotem GilboaGil Shoham
Feb 28, 2002·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·David L BienvenueRichard C Holz
Dec 12, 2002·Chemical Reviews·W Todd Lowther, Brian W Matthews
Apr 26, 2003·Current Opinion in Chemical Biology·Richard C HolzSabina I Swierczek
Jul 25, 2003·Applied Microbiology and Biotechnology·H Takagi, M Takahashi
Jul 29, 2004·FEBS Letters·Yifat Fundoiano-HershcovitzYuval Shoham
Sep 25, 2004·Acta Crystallographica. Section D, Biological Crystallography·Vera ReilandGil Shoham
Oct 20, 2005·Journal of Bioscience and Bioengineering·Toshikazu NishiwakiKiyoshi Hayashi

Citations

Jun 10, 2010·Bioscience, Biotechnology, and Biochemistry·Jiro ArimaNobuhiro Mori
Feb 22, 2013·Journal of the Science of Food and Agriculture·Xinxing GaoZhemin Zhou

Related Concepts

Aminopeptidase
Biotechnology
Streptomyces
Substrate Specificity

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